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Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues

Abstract

This study compared the gene expression of the immunoinflammatory markers interleukin (IL)-6, IL-1ß, and tumor necrosis factor alpha (TNF-α), the matrix metalloproteinases (MMP)-1, -2, -8, and -9, and the tissue inhibitors of matrix metalloproteases (TIMP)-1 and -2 in the gingival tissue of individuals with periodontal and peri-implant disease. The study population included individuals with four periodontal statuses: periodontal health (PH group, n = 20); periodontitis (P group, n = 20); peri-implant health (PIH group, n = 20), and peri-implantitis (PI group, n = 20). Gingival biopsies were collected from one tooth per patient according to the inclusion criteria of each group. The mRNA levels of IL-6, IL-1ß, TNF-α, MMP-1, MMP-2, MMP-8, MMP-9, TIMP-1, and TIMP-2 were evaluated by qPCR. The levels of IL-1ß were significantly higher in the PI group when compared to the other groups ( < 0.05), while the levels of IL-6 were significantly higher in the groups with periodontal and peri-implant disease when compared with the healthy groups ( < 0.05); however, the levels of IL-6 did not differ between the PI and P groups ( > 0.05). For all other studied biomarkers, no significant differences were observed between groups ( > 0.05). IL-6 and IL-1ß presented higher levels of mRNA in diseased periodontal and peri-implant tissues. However, the expression of metalloproteinases and their inhibitors did not differ between the different periodontal statuses.

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References
1.
Sorsa T, Hernandez M, Leppilahti J, Munjal S, Netuschil L, Mantyla P . Detection of gingival crevicular fluid MMP-8 levels with different laboratory and chair-side methods. Oral Dis. 2009; 16(1):39-45. DOI: 10.1111/j.1601-0825.2009.01603.x. View

2.
Marcaccini A, Meschiari C, Zuardi L, Sampaio de Sousa T, Taba Jr M, Teofilo J . Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy. J Clin Periodontol. 2009; 37(2):180-90. DOI: 10.1111/j.1600-051X.2009.01512.x. View

3.
Duarte P, Miranda T, Lima J, Goncalves T, Santos V, Bastos M . Expression of immune-inflammatory markers in sites of chronic periodontitis in patients with type 2 diabetes. J Periodontol. 2011; 83(4):426-34. DOI: 10.1902/jop.2011.110324. View

4.
Koch S, Volkmar C, Kolb-Bachofen V, Korth H, Kirsch M, Horn A . A new redox-dependent mechanism of MMP-1 activity control comprising reduced low-molecular-weight thiols and oxidizing radicals. J Mol Med (Berl). 2008; 87(3):261-72. DOI: 10.1007/s00109-008-0420-5. View

5.
Ramseier C, Eick S, Bronnimann C, Buser D, Bragger U, Salvi G . Host-derived biomarkers at teeth and implants in partially edentulous patients. A 10-year retrospective study. Clin Oral Implants Res. 2015; 27(2):211-7. DOI: 10.1111/clr.12566. View