TMEM150B is Dispensable for Oocyte Maturation and Female Fertility in Mouse

Overview

Journal Sci Rep

Specialty Science

Date 2020 Dec 8

PMID 33288838

Citations 1

Authors

Ran Liu

Hanni Ke

Tong Shao

Yingying Qin

Shidou Zhao

Affiliations

Soon will be listed here.

Abstract

Premature ovarian insufficiency (POI) refers to severe decline of ovary function in females which usually leads to infertility. It has been reported that the TMEM150B gene is mostly associated with age at natural menopause, early menopause and POI, but its role in female reproduction remains unknown. In this study, we found Tmem150b was highly expressed in mouse oocytes, but its deletion had no obvious effect on meiotic maturation of oocytes indicated by first polar body emission and spindle morphology. There were also no obvious differences in follicle development and corpus luteum formation between knockout and wild type mice. Finally, knockout of Tmem150b did not affect female fertility and sexual hormone levels. In summary, our results suggest that TMEM150B is not essential for female fertility in mice.

Citing Articles

Genetic Abnormalities of Oocyte Maturation: Mechanisms and Clinical Implications.

Baldini G, Ferri D, Malvasi A, Lagana A, Vimercati A, Dellino M Int J Mol Sci. 2024; 25(23).

PMID: 39684710 PMC: 11640808. DOI: 10.3390/ijms252313002.

References

Mrschtik M, Ryan K . Another DRAM involved in autophagy and cell death. Autophagy. 2016; 12(3):603-5. PMC: 4836028. DOI: 10.1080/15548627.2015.1137412. View

Shao T, Ke H, Liu R, Zhao S, Qin Y . Variation analysis of theTMEM150B gene in Chinese women with premature ovarian insufficiency. Reprod Biomed Online. 2019; 38(3):407-412. DOI: 10.1016/j.rbmo.2018.12.009. View

De Vos M, Devroey P, Fauser B . Primary ovarian insufficiency. Lancet. 2010; 376(9744):911-21. DOI: 10.1016/S0140-6736(10)60355-8. View

Alasiri S, Basit S, Wood-Trageser M, Yatsenko S, Jeffries E, Surti U . Exome sequencing reveals MCM8 mutation underlies ovarian failure and chromosomal instability. J Clin Invest. 2014; 125(1):258-62. PMC: 4382257. DOI: 10.1172/JCI78473. View

Nelson L . Clinical practice. Primary ovarian insufficiency. N Engl J Med. 2009; 360(6):606-14. PMC: 2762081. DOI: 10.1056/NEJMcp0808697. View

Mrschtik M, OPrey J, Lao L, Long J, Beaumatin F, Strachan D . DRAM-3 modulates autophagy and promotes cell survival in the absence of glucose. Cell Death Differ. 2015; 22(10):1714-26. PMC: 4563785. DOI: 10.1038/cdd.2015.26. View

Laisk-Podar T, Lindgren C, Peters M, Tapanainen J, Lambalk C, Salumets A . Ovarian Physiology and GWAS: Biobanks, Biology, and Beyond. Trends Endocrinol Metab. 2016; 27(7):516-528. PMC: 7610559. DOI: 10.1016/j.tem.2016.04.011. View

Tibiletti M, Testa G, Vegetti W, Alagna F, Taborelli M, Dalpra L . The idiopathic forms of premature menopause and early menopause show the same genetic pattern. Hum Reprod. 1999; 14(11):2731-4. DOI: 10.1093/humrep/14.11.2731. View

He W, Tan C, Zhang Y, Meng L, Gong F, Lu G . Homozygous variants in cause premature ovarian insufficiency. J Med Genet. 2020; 58(3):168-172. PMC: 7907585. DOI: 10.1136/jmedgenet-2019-106789. View

10.

Song Z, Yu H, Wang P, Mao G, Liu W, Li M . Germ cell-specific Atg7 knockout results in primary ovarian insufficiency in female mice. Cell Death Dis. 2015; 6:e1589. PMC: 4669757. DOI: 10.1038/cddis.2014.559. View

11.

Day F, Ruth K, Thompson D, Lunetta K, Pervjakova N, Chasman D . Large-scale genomic analyses link reproductive aging to hypothalamic signaling, breast cancer susceptibility and BRCA1-mediated DNA repair. Nat Genet. 2015; 47(11):1294-1303. PMC: 4661791. DOI: 10.1038/ng.3412. View

12.

Perry J, Corre T, Esko T, Chasman D, Fischer K, Franceschini N . A genome-wide association study of early menopause and the combined impact of identified variants. Hum Mol Genet. 2013; 22(7):1465-72. PMC: 3596848. DOI: 10.1093/hmg/dds551. View

13.

Jiao X, Ke H, Qin Y, Chen Z . Molecular Genetics of Premature Ovarian Insufficiency. Trends Endocrinol Metab. 2018; 29(11):795-807. DOI: 10.1016/j.tem.2018.07.002. View

14.

Wu X, Cai H, Kallianpur A, Li H, Yang G, Gao J . Impact of premature ovarian failure on mortality and morbidity among Chinese women. PLoS One. 2014; 9(3):e89597. PMC: 3945971. DOI: 10.1371/journal.pone.0089597. View

15.

Dou X, Guo T, Li G, Zhou L, Qin Y, Chen Z . Minichromosome maintenance complex component 8 mutations cause primary ovarian insufficiency. Fertil Steril. 2016; 106(6):1485-1489.e2. DOI: 10.1016/j.fertnstert.2016.08.018. View

16.

Zeng J, Jiang M, Wu X, Diao F, Qiu D, Hou X . SIRT4 is essential for metabolic control and meiotic structure during mouse oocyte maturation. Aging Cell. 2018; 17(4):e12789. PMC: 6052465. DOI: 10.1111/acel.12789. View

17.

Lutzmann M, Grey C, Traver S, Ganier O, Maya-Mendoza A, Ranisavljevic N . MCM8- and MCM9-deficient mice reveal gametogenesis defects and genome instability due to impaired homologous recombination. Mol Cell. 2012; 47(4):523-34. DOI: 10.1016/j.molcel.2012.05.048. View

18.

Zhou J, Stein P, Leu N, Chmatal L, Xue J, Ma J . Accelerated reproductive aging in females lacking a novel centromere protein SYCP2L. Hum Mol Genet. 2015; 24(22):6505-14. PMC: 4614708. DOI: 10.1093/hmg/ddv359. View

19.

Qin Y, Jiao X, Simpson J, Chen Z . Genetics of primary ovarian insufficiency: new developments and opportunities. Hum Reprod Update. 2015; 21(6):787-808. PMC: 4594617. DOI: 10.1093/humupd/dmv036. View

20.

Guo T, Zhao S, Zhao S, Chen M, Li G, Jiao X . Mutations in MSH5 in primary ovarian insufficiency. Hum Mol Genet. 2017; 26(8):1452-1457. PMC: 5393145. DOI: 10.1093/hmg/ddx044. View