Spectroscopic Detection of Traumatic Brain Injury Severity and Biochemistry from the Retina

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2020 Dec 7

PMID 33282487

Citations 12

Authors

Carl Banbury

Iain Styles

Neil Eisenstein

Elisa R Zanier

Gloria Vegliante

Antonio Belli

Ann Logan

Pola Goldberg Oppenheimer

Affiliations

Soon will be listed here.

Abstract

Traumatic brain injury (TBI) is a major burden on healthcare services worldwide, where scientific and clinical innovation is needed to provide better understanding of biochemical damage to improve both pre-hospital assessment and intensive care monitoring. Here, we present an unconventional concept of using Raman spectroscopy to measure the biochemical response to the retina in an murine model of TBI. Through comparison to spectra from the brain and retina following injury, we elicit subtle spectral changes through the use of multivariate analysis, linked to a decrease in cardiolipin and indicating metabolic disruption. The ability to classify injury severity via spectra of the retina is demonstrated for severe TBI (82.0 %), moderate TBI (75.1 %) and sham groups (69.4 %). By showing that optical spectroscopy can be used to explore the eye as the window to the brain, we lay the groundwork for further exploitation of Raman spectroscopy for indirect, non-invasive assessment of brain chemistry.

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