Development of a Visible Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for the Detection of Rift Valley Fever Virus

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2020 Dec 7

PMID 33281787

Citations 10

Authors

Qiuxue Han

Shengnan Zhang

Dongping Liu

Feihu Yan

Hualei Wang

Pei Huang

Jinhao Bi

Hongli Jin

Na Feng

Zengguo Cao

Yuwei Gao

Hang Chi

Songtao Yang

Yongkun Zhao

Xianzhu Xia

Affiliations

Soon will be listed here.

Abstract

Rift Valley fever (RVF) is a severe infectious disease, which can through mosquito bites, direct contact and aerosol transmission infect sheep, goats, people, camels, cattle, buffaloes, and so on. In this paper, a conserved region of the S RNA segment of Rift Valley fever virus (RVFV) ZH501 strain was used as target sequence. The RVFV RT-LAMP-VF assay was successfully established combined reverse transcription-loop-mediated isothermal amplification with a vertical flow visualization strip. The detection limit is up to 1.94 × 10 copies/μl of synthesized RVFV-RNA. RNA extracted from cell culture of an inactivated RVFV-BJ01 strain was also used as templates, and the detection limit is 1.83 × 10 copies/μl. In addition, there was no cross-reactivity with other viruses that can cause similar fever symptoms. The RVFV-LAMP-VF assay exhibited very high levels of diagnostic sensitivity, which had 100-fold more sensitive than RVFV real-time RT-PCR assay. Accordingly, the RVFV RT-LAMP-VF assay developed in this study is suitable for the rapid and sensitive diagnosis of RVFV without specialized equipment and can rapidly complete detection within 60 min, and the results are visible by vertical flow visualization strip within 5 min.

Citing Articles

Evolving Landscape of Emerging Virus Diagnosis: Challenges and Innovations.

Kumar A, Saini S, Anvikar A, Mishra N, Misra G Mol Biotechnol. 2025; .

PMID: 40042766 DOI: 10.1007/s12033-025-01385-w.

Molecular Diagnosis of Phlebovirus riftense Infection Using an L-Segment-Based Real-Time RT-PCR Method.

Pedarrieu A, Cetre-Sossah C Methods Mol Biol. 2024; 2893:37-49.

PMID: 39671028 DOI: 10.1007/978-1-0716-4338-9_4.

Development of a label-free electrochemical aptasensor for Rift Valley fever virus detection.

AlHarbi M, Rhouati A, Zourob M Sci Rep. 2024; 14(1):23892.

PMID: 39396078 PMC: 11470950. DOI: 10.1038/s41598-024-74314-7.

Detection and Diagnosis of Rift Valley Fever Virus.

Bob N, Dia M, Ndiaye O, Ba A, Prudhomme J, Diagne M Methods Mol Biol. 2024; 2824:35-65.

PMID: 39039405 DOI: 10.1007/978-1-0716-3926-9_4.

Rift Valley Fever Virus: An Overview of the Current Status of Diagnostics.

Lapa D, Pauciullo S, Ricci I, Garbuglia A, Maggi F, Scicluna M Biomedicines. 2024; 12(3).

PMID: 38540153 PMC: 10968371. DOI: 10.3390/biomedicines12030540.

References

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N . Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000; 28(12):E63. PMC: 102748. DOI: 10.1093/nar/28.12.e63. View

Paweska J, van Vuren P, Kemp A, Buss P, Bengis R, Gakuya F . Recombinant nucleocapsid-based ELISA for detection of IgG antibody to Rift Valley fever virus in African buffalo. Vet Microbiol. 2007; 127(1-2):21-8. DOI: 10.1016/j.vetmic.2007.07.031. View

Ikegami T, Makino S . The pathogenesis of Rift Valley fever. Viruses. 2011; 3(5):493-519. PMC: 3111045. DOI: 10.3390/v3050493. View

Mori N, Motegi Y, Shimamura Y, Ezaki T, Natsumeda T, Yonekawa T . Development of a new method for diagnosis of rubella virus infection by reverse transcription-loop-mediated isothermal amplification. J Clin Microbiol. 2006; 44(9):3268-73. PMC: 1594746. DOI: 10.1128/JCM.00803-06. View

Kurosaki Y, Martins D, Kimura M, Dos Santos Catena A, Borba M, Mattos S . Development and evaluation of a rapid molecular diagnostic test for Zika virus infection by reverse transcription loop-mediated isothermal amplification. Sci Rep. 2017; 7(1):13503. PMC: 5647432. DOI: 10.1038/s41598-017-13836-9. View

Madani T, Al-Mazrou Y, Al-Jeffri M, Mishkhas A, Al-Rabeah A, Turkistani A . Rift Valley fever epidemic in Saudi Arabia: epidemiological, clinical, and laboratory characteristics. Clin Infect Dis. 2003; 37(8):1084-92. DOI: 10.1086/378747. View

Wichgers Schreur P, Paweska J, Kant J, Kortekaas J . A novel highly sensitive, rapid and safe Rift Valley fever virus neutralization test. J Virol Methods. 2017; 248:26-30. DOI: 10.1016/j.jviromet.2017.06.001. View

Mansfield K, Banyard A, McElhinney L, Johnson N, Horton D, Hernandez-Triana L . Rift Valley fever virus: A review of diagnosis and vaccination, and implications for emergence in Europe. Vaccine. 2015; 33(42):5520-5531. DOI: 10.1016/j.vaccine.2015.08.020. View

Hong T, Mai Q, Cuong D, Parida M, Minekawa H, Notomi T . Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus. J Clin Microbiol. 2004; 42(5):1956-61. PMC: 404656. DOI: 10.1128/JCM.42.5.1956-1961.2004. View

10.

Garcia S, Crance J, Billecocq A, Peinnequin A, Jouan A, Bouloy M . Quantitative real-time PCR detection of Rift Valley fever virus and its application to evaluation of antiviral compounds. J Clin Microbiol. 2001; 39(12):4456-61. PMC: 88565. DOI: 10.1128/JCM.39.12.4456-4461.2001. View

11.

Peyrefitte C, Boubis L, Coudrier D, Bouloy M, Grandadam M, Tolou H . Real-time reverse-transcription loop-mediated isothermal amplification for rapid detection of rift valley Fever virus. J Clin Microbiol. 2008; 46(11):3653-9. PMC: 2576582. DOI: 10.1128/JCM.01188-08. View

12.

Euler M, Wang Y, Nentwich O, Piepenburg O, Hufert F, Weidmann M . Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus. J Clin Virol. 2012; 54(4):308-12. DOI: 10.1016/j.jcv.2012.05.006. View

13.

Okamoto S, Yoshikawa T, Ihira M, Suzuki K, Shimokata K, Nishiyama Y . Rapid detection of varicella-zoster virus infection by a loop-mediated isothermal amplification method. J Med Virol. 2004; 74(4):677-82. DOI: 10.1002/jmv.20223. View

14.

Sobarzo A, Paweska J, Herrmann S, Amir T, Marks R, Lobel L . Optical fiber immunosensor for the detection of IgG antibody to Rift Valley fever virus in humans. J Virol Methods. 2007; 146(1-2):327-34. DOI: 10.1016/j.jviromet.2007.07.017. View

15.

Ragan I, Davis A, McVey D, Richt J, Rowland R, Wilson W . Evaluation of Fluorescence Microsphere Immunoassay for Detection of Antibodies to Rift Valley Fever Virus Nucleocapsid Protein and Glycoproteins. J Clin Microbiol. 2018; 56(6). PMC: 5971554. DOI: 10.1128/JCM.01626-17. View

16.

Huang P, Wang H, Cao Z, Jin H, Chi H, Zhao J . A Rapid and Specific Assay for the Detection of MERS-CoV. Front Microbiol. 2018; 9:1101. PMC: 5987675. DOI: 10.3389/fmicb.2018.01101. View

17.

van Vuren P, Potgieter A, Paweska J, van Dijk A . Preparation and evaluation of a recombinant Rift Valley fever virus N protein for the detection of IgG and IgM antibodies in humans and animals by indirect ELISA. J Virol Methods. 2006; 140(1-2):106-14. DOI: 10.1016/j.jviromet.2006.11.005. View

18.

Rolin A, Berrang-Ford L, Kulkarni M . The risk of Rift Valley fever virus introduction and establishment in the United States and European Union. Emerg Microbes Infect. 2015; 2(12):e81. PMC: 3880870. DOI: 10.1038/emi.2013.81. View

19.

Upreti D, Cernicchiaro N, Richt J, Wilson W, Clavijo A, Davis A . Preliminary evaluation of diagnostic accuracy and precision of a competitive ELISA for detection of antibodies to Rift Valley fever virus in cattle and sheep sera. J Virol Methods. 2018; 262:6-11. DOI: 10.1016/j.jviromet.2018.09.002. View

20.

Elliott R . Emerging viruses: the Bunyaviridae. Mol Med. 1997; 3(9):572-7. PMC: 2230091. View