How Have Leukocyte In Vitro Chemotaxis Assays Shaped Our Ideas About Macrophage Migration?

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2020 Dec 5

PMID 33276594

Citations 9

Authors

Agata N Rumianek

David R Greaves

Affiliations

Soon will be listed here.

Abstract

Macrophage chemotaxis is crucial during both onset and resolution of inflammation and unique among all leukocytes. Macrophages are able to switch between amoeboid and mesenchymal migration to optimise their migration through 3D environments. This subtle migration phenotype has been underappreciated in the literature, with macrophages often being grouped and discussed together with other leukocytes, possibly due to the limitations of current chemotaxis assays. Transwell assays were originally designed in the 1960s but despite their long-known limitations, they are still one of the most popular methods of studying macrophage migration. This review aims to critically evaluate transwell assays, and other popular chemotaxis assays, comparing their advantages and limitations in macrophage migration studies.

Citing Articles

Ly6C Monocytes Are Metabolically Reprogrammed in the Blood during Inflammatory Stimulation and Require Intact OxPhos for Chemotaxis and Monocyte to Macrophage Differentiation.

Purvis G, McNeill E, Wright B, Channon K, Greaves D Cells. 2024; 13(11.

PMID: 38891050 PMC: 11171939. DOI: 10.3390/cells13110916.

A vascularized melanoma model suitable for metastasis research of different tumor stages using fundamentally different bioinks.

Schmid R, Schmidt S, Schrufer S, Schubert D, Heltmann-Meyer S, Schicht M Mater Today Bio. 2024; 26:101071.

PMID: 38736612 PMC: 11081803. DOI: 10.1016/j.mtbio.2024.101071.

Markers of the ageing macrophage: a systematic review and meta-analysis.

Moss C, Phipps H, Wilson H, Kiss-Toth E Front Immunol. 2023; 14:1222308.

PMID: 37520567 PMC: 10373068. DOI: 10.3389/fimmu.2023.1222308.

Assaying Microglia Functions In Vitro.

Maguire E, Connor-Robson N, Shaw B, ODonoghue R, Stoberl N, Hall-Roberts H Cells. 2022; 11(21).

PMID: 36359810 PMC: 9654693. DOI: 10.3390/cells11213414.

β2-Integrins - Regulatory and Executive Bridges in the Signaling Network Controlling Leukocyte Trafficking and Migration.

Guenther C Front Immunol. 2022; 13:809590.

PMID: 35529883 PMC: 9072638. DOI: 10.3389/fimmu.2022.809590.

References

Harris H . Chemotaxis of granulocytes. J Pathol Bacteriol. 1953; 66(1):135-46. DOI: 10.1002/path.1700660117. View

Vianello F, Olszak I, Poznansky M . Fugetaxis: active movement of leukocytes away from a chemokinetic agent. J Mol Med (Berl). 2005; 83(10):752-63. DOI: 10.1007/s00109-005-0675-z. View

Frow E, Reckless J, Grainger D . Tools for anti-inflammatory drug design: in vitro models of leukocyte migration. Med Res Rev. 2004; 24(3):276-98. DOI: 10.1002/med.10062. View

Epelman S, Lavine K, Randolph G . Origin and functions of tissue macrophages. Immunity. 2014; 41(1):21-35. PMC: 4470379. DOI: 10.1016/j.immuni.2014.06.013. View

Taylor L, Brodermann M, McCaffary D, Iqbal A, Greaves D . Netrin-1 Reduces Monocyte and Macrophage Chemotaxis towards the Complement Component C5a. PLoS One. 2016; 11(8):e0160685. PMC: 4980032. DOI: 10.1371/journal.pone.0160685. View

Falk W, Goodwin Jr R, Leonard E . A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration. J Immunol Methods. 1980; 33(3):239-47. DOI: 10.1016/0022-1759(80)90211-2. View

Trepat X, Chen Z, Jacobson K . Cell migration. Compr Physiol. 2013; 2(4):2369-92. PMC: 4457291. DOI: 10.1002/cphy.c110012. View

Cui K, Ardell C, Podolnikova N, Yakubenko V . Distinct Migratory Properties of M1, M2, and Resident Macrophages Are Regulated by αβ and αβ Integrin-Mediated Adhesion. Front Immunol. 2018; 9:2650. PMC: 6262406. DOI: 10.3389/fimmu.2018.02650. View

Boneschansker L, Nakayama H, Eisenga M, Wedel J, Klagsbrun M, Irimia D . Netrin-1 Augments Chemokinesis in CD4+ T Cells In Vitro and Elicits a Proinflammatory Response In Vivo. J Immunol. 2016; 197(4):1389-98. PMC: 4976028. DOI: 10.4049/jimmunol.1502432. View

10.

McDonald B, Pittman K, Menezes G, Hirota S, Slaba I, Waterhouse C . Intravascular danger signals guide neutrophils to sites of sterile inflammation. Science. 2010; 330(6002):362-6. DOI: 10.1126/science.1195491. View

11.

Penzo M, Habiel D, Ramadass M, Kew R, Marcu K . Cell migration to CXCL12 requires simultaneous IKKα and IKKβ-dependent NF-κB signaling. Biochim Biophys Acta. 2014; 1843(9):1796-1804. PMC: 4096130. DOI: 10.1016/j.bbamcr.2014.04.011. View

12.

Wolf K, Muller R, Borgmann S, Brocker E, Friedl P . Amoeboid shape change and contact guidance: T-lymphocyte crawling through fibrillar collagen is independent of matrix remodeling by MMPs and other proteases. Blood. 2003; 102(9):3262-9. DOI: 10.1182/blood-2002-12-3791. View

13.

Rink I, Rink J, Helmer D, Sachs D, Schmitz K . A haptotaxis assay for leukocytes based on surface-bound chemokine gradients. J Immunol. 2015; 194(11):5549-58. DOI: 10.4049/jimmunol.1500148. View

14.

Zicha D, Dunn G, Brown A . A new direct-viewing chemotaxis chamber. J Cell Sci. 1991; 99 ( Pt 4):769-75. DOI: 10.1242/jcs.99.4.769. View

15.

van Goethem E, Poincloux R, Gauffre F, Maridonneau-Parini I, Le Cabec V . Matrix architecture dictates three-dimensional migration modes of human macrophages: differential involvement of proteases and podosome-like structures. J Immunol. 2009; 184(2):1049-61. DOI: 10.4049/jimmunol.0902223. View

16.

Barros-Becker F, Lam P, Fisher R, Huttenlocher A . Live imaging reveals distinct modes of neutrophil and macrophage migration within interstitial tissues. J Cell Sci. 2017; 130(22):3801-3808. PMC: 5702045. DOI: 10.1242/jcs.206128. View

17.

Renkawitz J, Kopf A, Stopp J, de Vries I, Driscoll M, Merrin J . Nuclear positioning facilitates amoeboid migration along the path of least resistance. Nature. 2019; 568(7753):546-550. PMC: 7217284. DOI: 10.1038/s41586-019-1087-5. View

18.

Gervais F, Cruz R, Chateauneuf A, Gale S, Sawyer N, Nantel F . Selective modulation of chemokinesis, degranulation, and apoptosis in eosinophils through the PGD2 receptors CRTH2 and DP. J Allergy Clin Immunol. 2001; 108(6):982-8. DOI: 10.1067/mai.2001.119919. View

19.

Debru C . A particular form of chemotaxis: necrotaxis. An historical view. Blood Cells. 1993; 19(1):5-19; discussion 20-3. View

20.

Pakshir P, Alizadehgiashi M, Wong B, Coelho N, Chen X, Gong Z . Dynamic fibroblast contractions attract remote macrophages in fibrillar collagen matrix. Nat Commun. 2019; 10(1):1850. PMC: 6478854. DOI: 10.1038/s41467-019-09709-6. View