Characterization of a Novel Yeast Phase-specific Antigen Expressed During in Vitro Thermal Phase Transition of Talaromyces Marneffei

Overview

Journal Sci Rep

Specialty Science

Date 2020 Dec 4

PMID 33273617

Citations 12

Authors

Kritsada Pruksaphon

Mc Millan Nicol Ching

Joshua D Nosanchuk

Anna Kaltsas

Kavi Ratanabanangkoon

Sittiruk Roytrakul

Luis R Martinez

Sirida Youngchim

Affiliations

Soon will be listed here.

Abstract

Talaromyces marneffei is a dimorphic fungus that has emerged as an opportunistic pathogen particularly in individuals with HIV/AIDS. Since its dimorphism has been associated with its virulence, the transition from mold to yeast-like cells might be important for fungal pathogenesis, including its survival inside of phagocytic host cells. We investigated the expression of yeast antigen of T. marneffei using a yeast-specific monoclonal antibody (MAb) 4D1 during phase transition. We found that MAb 4D1 recognizes and binds to antigenic epitopes on the surface of yeast cells. Antibody to antigenic determinant binding was associated with time of exposure, mold to yeast conversion, and mammalian temperature. We also demonstrated that MAb 4D1 binds to and recognizes conidia to yeast cells' transition inside of a human monocyte-like THP-1 cells line. Our studies are important because we demonstrated that MAb 4D1 can be used as a tool to study T. marneffei virulence, furthering the understanding of the therapeutic potential of passive immunity in this fungal pathogenesis.

Citing Articles

An unusual Toll/MyD88-mediated host defence against .

Wang X, Qu Q, Li Z, Lu S, Ferrandon D, Xi L Fly (Austin). 2024; 18(1):2398300.

PMID: 39239739 PMC: 11382710. DOI: 10.1080/19336934.2024.2398300.

The microbial damage and host response framework: lesson learned from pathogenic survival trajectories and immunoinflammatory responses of infection.

Pruksaphon K, Amsri A, Jeenkeawpieam J, Thammasit P, Nosanchuk J, Youngchim S Front Immunol. 2024; 15:1448729.

PMID: 39188728 PMC: 11345217. DOI: 10.3389/fimmu.2024.1448729.

Talaromyces marneffei suppresses macrophage inflammation by regulating host alternative splicing.

Wei W, Wang G, Zhang H, Bao X, An S, Luo Q Commun Biol. 2023; 6(1):1046.

PMID: 37845378 PMC: 10579421. DOI: 10.1038/s42003-023-05409-6.

Evaluation of the yeast phase-specific monoclonal antibody 4D1 and agglutinin sandwich ELISA to detect antigen in human urine.

Shu F, Pruksaphon K, Nosanchuk J, Thammasit P, Youngchim S Front Cell Infect Microbiol. 2023; 13:1163868.

PMID: 37705932 PMC: 10495985. DOI: 10.3389/fcimb.2023.1163868.

Extracellular vesicles derived from contain immunogenic compounds and modulate THP-1 macrophage responses.

Pruksaphon K, Amsri A, Thammasit P, Nosanchuk J, Youngchim S Front Immunol. 2023; 14:1192326.

PMID: 37457708 PMC: 10339390. DOI: 10.3389/fimmu.2023.1192326.

References

Pruksaphon K, Intaramat A, Ratanabanangkoon K, Nosanchuk J, Vanittanakom N, Youngchim S . Development and characterization of an immunochromatographic test for the rapid diagnosis of Talaromyces (Penicillium) marneffei. PLoS One. 2018; 13(4):e0195596. PMC: 5895032. DOI: 10.1371/journal.pone.0195596. View

San-Blas G, Travassos L, Fries B, Goldman D, Casadevall A, Carmona A . Fungal morphogenesis and virulence. Med Mycol. 2001; 38 Suppl 1:79-86. View

Pruksaphon K, Intaramat A, Ratanabanangkoon K, Nosanchuk J, Vanittanakom N, Youngchim S . Diagnostic laboratory immunology for talaromycosis (penicilliosis): review from the bench-top techniques to the point-of-care testing. Diagn Microbiol Infect Dis. 2019; 96(3):114959. DOI: 10.1016/j.diagmicrobio.2019.114959. View

Tsang C, Lau S, Woo P . Sixty Years from Segretain's Description: What Have We Learned and Should Learn About the Basic Mycology of Talaromyces marneffei?. Mycopathologia. 2019; 184(6):721-729. DOI: 10.1007/s11046-019-00395-y. View

Dong R, Zhang Y, Zhu L, Liu H, Liu J, Kuang Y . Innate Immunity Acts as the Major Regulator in Coinfected AIDS Patients: Cytokine Profile Surveillance During Initial 6-Month Antifungal Therapy. Open Forum Infect Dis. 2019; 6(6):ofz205. PMC: 6559339. DOI: 10.1093/ofid/ofz205. View

Shibuya N, Goldstein I, Van Damme E, Peumans W . Binding properties of a mannose-specific lectin from the snowdrop (Galanthus nivalis) bulb. J Biol Chem. 1988; 263(2):728-34. View

Diez S, Gomez B, Restrepo A, Hay R, Hamilton A . Paracoccidioides brasiliensis 87-kilodalton antigen, a heat shock protein useful in diagnosis: characterization, purification, and detection in biopsy material via immunohistochemistry. J Clin Microbiol. 2002; 40(2):359-65. PMC: 153377. DOI: 10.1128/JCM.40.2.359-365.2002. View

Guimaraes A, Nakayasu E, Sobreira T, Cordero R, Nimrichter L, Almeida I . Histoplasma capsulatum heat-shock 60 orchestrates the adaptation of the fungus to temperature stress. PLoS One. 2011; 6(2):e14660. PMC: 3037374. DOI: 10.1371/journal.pone.0014660. View

Boyce K, Andrianopoulos A . Fungal dimorphism: the switch from hyphae to yeast is a specialized morphogenetic adaptation allowing colonization of a host. FEMS Microbiol Rev. 2015; 39(6):797-811. DOI: 10.1093/femsre/fuv035. View

10.

Rimek D, Singh J, Kappe R . Cross-reactivity of the PLATELIA CANDIDA antigen detection enzyme immunoassay with fungal antigen extracts. J Clin Microbiol. 2003; 41(7):3395-8. PMC: 165363. DOI: 10.1128/JCM.41.7.3395-3398.2003. View

11.

Srinoulprasert Y, Pongtanalert P, Chawengkirttikul R, Chaiyaroj S . Engagement of Penicillium marneffei conidia with multiple pattern recognition receptors on human monocytes. Microbiol Immunol. 2009; 53(3):162-72. DOI: 10.1111/j.1348-0421.2008.00102.x. View

12.

Marcos C, de Oliveira H, de Melo W, da Silva J, Assato P, Scorzoni L . Anti-Immune Strategies of Pathogenic Fungi. Front Cell Infect Microbiol. 2016; 6:142. PMC: 5108756. DOI: 10.3389/fcimb.2016.00142. View

13.

KANETSUNA F, CARBONELL L, MORENO R, Rodriguez J . Cell wall composition of the yeast and mycelial forms of Paracoccidioides brasiliensis. J Bacteriol. 1969; 97(3):1036-41. PMC: 249811. DOI: 10.1128/jb.97.3.1036-1041.1969. View

14.

Prakit K, Nosanchuk J, Pruksaphon K, Vanittanakom N, Youngchim S . A novel inhibition ELISA for the detection and monitoring of Penicillium marneffei antigen in human serum. Eur J Clin Microbiol Infect Dis. 2016; 35(4):647-56. DOI: 10.1007/s10096-016-2583-2. View

15.

Andrianopoulos A . Control of morphogenesis in the human fungal pathogen Penicillium marneffei. Int J Med Microbiol. 2002; 292(5-6):331-47. DOI: 10.1078/1438-4221-00217. View

16.

Dean N . Asparagine-linked glycosylation in the yeast Golgi. Biochim Biophys Acta. 1999; 1426(2):309-22. DOI: 10.1016/s0304-4165(98)00132-9. View

17.

Boyce K, Andrianopoulos A . Morphogenetic circuitry regulating growth and development in the dimorphic pathogen Penicillium marneffei. Eukaryot Cell. 2012; 12(2):154-60. PMC: 3571290. DOI: 10.1128/EC.00234-12. View

18.

Cao L, Chan C, Lee C, Wong S, Yuen K . MP1 encodes an abundant and highly antigenic cell wall mannoprotein in the pathogenic fungus Penicillium marneffei. Infect Immun. 1998; 66(3):966-73. PMC: 108003. DOI: 10.1128/IAI.66.3.966-973.1998. View

19.

Batista W, Matsuo A, Ganiko L, Barros T, Veiga T, Freymuller E . The PbMDJ1 gene belongs to a conserved MDJ1/LON locus in thermodimorphic pathogenic fungi and encodes a heat shock protein that localizes to both the mitochondria and cell wall of Paracoccidioides brasiliensis. Eukaryot Cell. 2006; 5(2):379-90. PMC: 1405898. DOI: 10.1128/EC.5.2.379-390.2006. View

20.

Vanittanakom N, Pongpom M, Praparattanapan J, Cooper C, Sirisanthana T . Isolation and expression of heat shock protein 30 gene from Penicillium marneffei. Med Mycol. 2009; 47(5):521-6. DOI: 10.1080/13693780802566358. View