A Bacteriocin-based Antimicrobial Formulation to Effectively Disrupt the Cell Viability of Methicillin-resistant Staphylococcus Aureus (MRSA) Biofilms

Overview

Journal NPJ Biofilms Microbiomes

Date 2020 Dec 3

PMID 33268776

Citations 26

Authors

Christian Kranjec

Kirill V Ovchinnikov

Torstein Gronseth

Kumar Ebineshan

Aparna Srikantam

Dzung B Diep

Affiliations

Soon will be listed here.

Abstract

Antibiotic-resistant and biofilm-associated infections brought about by methicillin-resistant Staphylococcus aureus (MRSA) strains is a pressing issue both inside as well as outside nosocomial environments worldwide. Here, we show that a combination of two bacteriocins with distinct structural and functional characteristics, garvicin KS, and micrococcin P1, showed a synergetic antibacterial activity against biofilms produced in vitro by S. aureus, including several MRSA strains. In addition, this bacteriocin-based antimicrobial combination showed the ability to restore the sensitivity of the highly resilient MRSA strain ATCC 33591 to the β-lactam antibiotic penicillin G. By using a combination of bacterial cell metabolic assays, confocal and scanning electron microscopy, we show that the combination between garvicin KS, micrococcin P1, and penicillin G potently inhibit cell viability within S. aureus biofilms by causing severe cell damage. Together these data indicate that bacteriocins can be valuable therapeutic tools in the fight against biofilm-associated MRSA infections.

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