Resveratrol Promotes the Survival and Neuronal Differentiation of Hypoxia-conditioned Neuronal Progenitor Cells in Rats with Cerebral Ischemia

Overview

Journal Front Med

Specialty General Medicine

Date 2020 Dec 2

PMID 33263836

Citations 9

Authors

Yao Yao

Rui Zhou

Rui Bai

Jing Wang

Mengjiao Tu

Jingjing Shi

Xiao He

Jinyun Zhou

Liu Feng

Yuanxue Gao

Fahuan Song

Feng Lan

Xingguo Liu

Mei Tian

Hong Zhang

Affiliations

Soon will be listed here.

Abstract

Hypoxia conditioning could increase the survival of transplanted neuronal progenitor cells (NPCs) in rats with cerebral ischemia but could also hinder neuronal differentiation partly by suppressing mitochondrial metabolism. In this work, the mitochondrial metabolism of hypoxia-conditioned NPCs (hcNPCs) was upregulated via the additional administration of resveratrol, an herbal compound, to resolve the limitation of hypoxia conditioning on neuronal differentiation. Resveratrol was first applied during the in vitro neuronal differentiation of hcNPCs and concurrently promoted the differentiation, synaptogenesis, and functional development of neurons derived from hcNPCs and restored the mitochondrial metabolism. Furthermore, this herbal compound was used as an adjuvant during hcNPC transplantation in a photothrombotic stroke rat model. Resveratrol promoted neuronal differentiation and increased the long-term survival of transplanted hcNPCs. 18-fluorine fluorodeoxyglucose positron emission tomography and rotarod test showed that resveratrol and hcNPC transplantation synergistically improved the neurological and metabolic recovery of stroke rats. In conclusion, resveratrol promoted the neuronal differentiation and therapeutic efficiency of hcNPCs in stroke rats via restoring mitochondrial metabolism. This work suggested a novel approach to promote the clinical translation of NPC transplantation therapy.

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