Diversity of Isolated from Date Palms Rhizosphere and Saline Environments: Isolation, Identification and Biological Activity Evaluation

Overview

Journal Microorganisms

Specialty Microbiology

Date 2020 Dec 1

PMID 33255541

Citations 9

Authors

Omar Messaoudi

Joachim Wink

Mourad Bendahou

Affiliations

Soon will be listed here.

Abstract

The diversity of cultural in two types of Algerian Sahara environments, including saline environments and date palms rhizosphere, was investigated. In this study, a total of 40 strains of actinomycetes was isolated from different soil samples, using a rehydration and centrifugation method. Molecular identification, based on 16S rRNA gene sequence analysis, revealed that these isolates were affiliated to six clusters corresponding to eight genera, including , and . A taxonomic analysis, based on the morphological, physiological, biochemical, and molecular investigation, of selected strains, which belong to the rare , was undertaken. Four strains (CG3, A111, A93, and A79) were found to form distinct phyletic lines and represent new actinobacterial taxa. An assessment of antimicrobial proprieties of the 40 obtained actinomycetes strains, showed moderate to strong antimicrobial activities against fungi and bacteria. This study demonstrated the richness of Algerian Sahara with rare , which can provide novel bioactive metabolites, to solving some of the most challenging problems of the day, such as multi-drug resistance.

Citing Articles

Isolation of Actinobacteria from Date Palm Rhizosphere with Enzymatic, Antimicrobial, Antioxidant, and Protein Denaturation Inhibitory Activities.

Smati M, Bramki A, Makhlouf F, Djebaili R, Farda B, Abdelhadi F Biomolecules. 2025; 15(1).

PMID: 39858459 PMC: 11764267. DOI: 10.3390/biom15010065.

Genomic, Molecular Networking-Based Metabolomic, and Bioactivity Profiling of Actinobacteria from Undisturbed Caves in Pakistan.

Nawaz S, Skala L, Amin M, Iruegas-Bocardo F, Samadi A, Zaman K Appl Biochem Biotechnol. 2025; .

PMID: 39786631 DOI: 10.1007/s12010-024-05158-0.

Exploring Bioactive Potential of WAE1 from Wadi El-Natron, Egypt.

Osman M, Abo-Elnasr A, Mohamed E Indian J Microbiol. 2024; 64(4):1685-1696.

PMID: 39678948 PMC: 11645374. DOI: 10.1007/s12088-024-01215-8.

Morphological diversity of actinobacteria isolated from oil palm compost (Elaeis guineensis).

Uesugi J, Dos Santos Caldas D, Coelho B, Prazes M, Omura L, Pismel J Braz J Microbiol. 2023; 55(1):455-469.

PMID: 38010583 PMC: 10920546. DOI: 10.1007/s42770-023-01178-w.

Characterization of Silver Carbonate Nanoparticles Biosynthesized Using Marine and Exploring of Their Antimicrobial and Antibiofilm Activity.

Messaoudi O, Benamar I, Azizi A, Albukhaty S, Khane Y, Sulaiman G Mar Drugs. 2023; 21(10).

PMID: 37888471 PMC: 10608482. DOI: 10.3390/md21100536.

References

Thornton A, Jose S, Bhagani S, Chadwick D, Dunn D, Gilson R . Hepatitis B, hepatitis C, and mortality among HIV-positive individuals. AIDS. 2017; 31(18):2525-2532. PMC: 5690308. DOI: 10.1097/QAD.0000000000001646. View

Boudjella H, Zitouni A, Coppel Y, Mathieu F, Monje M, Sabaou N . Antibiotic R2, a new angucyclinone compound from Streptosporangium sp. Sg3. J Antibiot (Tokyo). 2010; 63(12):709-11. DOI: 10.1038/ja.2010.111. View

Tian S, Pu X, Luo G, Zhao L, Xu L, Li W . Isolation and characterization of new p-Terphenyls with antifungal, antibacterial, and antioxidant activities from halophilic actinomycete Nocardiopsis gilva YIM 90087. J Agric Food Chem. 2013; 61(12):3006-12. DOI: 10.1021/jf400718w. View

cihak M, Kamenik Z, Smidova K, Bergman N, Benada O, Kofronova O . Secondary Metabolites Produced during the Germination of . Front Microbiol. 2018; 8:2495. PMC: 5733532. DOI: 10.3389/fmicb.2017.02495. View

Adam D, Maciejewska M, Naome A, Martinet L, Coppieters W, Karim L . Isolation, Characterization, and Antibacterial Activity of Hard-to-Culture Actinobacteria from Cave Moonmilk Deposits. Antibiotics (Basel). 2018; 7(2). PMC: 6023089. DOI: 10.3390/antibiotics7020028. View

Hayakawa M, Otoguro M, Takeuchi T, Yamazaki T, Iimura Y . Application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter. Antonie Van Leeuwenhoek. 2001; 78(2):171-85. DOI: 10.1023/a:1026579426265. View

Han K, Wu X, Jiang C, Huang R, Li Z, Feng T . Three New Compounds from the Actinomycete Actinocorallia aurantiaca. Nat Prod Bioprospect. 2019; 9(5):351-354. PMC: 6814668. DOI: 10.1007/s13659-019-00217-0. View

Sherwood E, Bibb M . The antibiotic planosporicin coordinates its own production in the actinomycete Planomonospora alba. Proc Natl Acad Sci U S A. 2013; 110(27):E2500-9. PMC: 3704008. DOI: 10.1073/pnas.1305392110. View

Subramani R, Sipkema D . Marine Rare Actinomycetes: A Promising Source of Structurally Diverse and Unique Novel Natural Products. Mar Drugs. 2019; 17(5). PMC: 6562664. DOI: 10.3390/md17050249. View

10.

Tyurin A, Alferova V, Korshun V . Chemical Elicitors of Antibiotic Biosynthesis in Actinomycetes. Microorganisms. 2018; 6(2). PMC: 6027282. DOI: 10.3390/microorganisms6020052. View

11.

Tata S, Aouiche A, Bijani C, Bouras N, Pont F, Mathieu F . Mzabimycins A and B, novel intracellular angucycline antibiotics produced by sp. PAL114 in synthetic medium containing L-tryptophan. Saudi Pharm J. 2020; 27(7):907-913. PMC: 6978613. DOI: 10.1016/j.jsps.2019.06.004. View

12.

Pachori P, Gothalwal R, Gandhi P . Emergence of antibiotic resistance in intensive care unit; a critical review. Genes Dis. 2019; 6(2):109-119. PMC: 6545445. DOI: 10.1016/j.gendis.2019.04.001. View

13.

Rutledge P, Challis G . Discovery of microbial natural products by activation of silent biosynthetic gene clusters. Nat Rev Microbiol. 2015; 13(8):509-23. DOI: 10.1038/nrmicro3496. View

14.

Dhakal D, Pokhrel A, Shrestha B, Sohng J . Marine Rare Actinobacteria: Isolation, Characterization, and Strategies for Harnessing Bioactive Compounds. Front Microbiol. 2017; 8:1106. PMC: 5471306. DOI: 10.3389/fmicb.2017.01106. View

15.

Siddharth S, Rai V R . Isolation and characterization of bioactive compounds with antibacterial, antioxidant and enzyme inhibitory activities from marine-derived rare actinobacteria, Nocardiopsis sp. SCA21. Microb Pathog. 2019; 137:103775. DOI: 10.1016/j.micpath.2019.103775. View

16.

Ding T, Yang L, Zhang W, Shen Y . The secondary metabolites of rare actinomycetes: chemistry and bioactivity. RSC Adv. 2022; 9(38):21964-21988. PMC: 9067109. DOI: 10.1039/c9ra03579f. View

17.

Baral B, Akhgari A, Metsa-Ketela M . Activation of microbial secondary metabolic pathways: Avenues and challenges. Synth Syst Biotechnol. 2018; 3(3):163-178. PMC: 6190515. DOI: 10.1016/j.synbio.2018.09.001. View

18.

Li W, Kroppenstedt R, Wang D, Tang S, Lee J, Park D . Five novel species of the genus Nocardiopsis isolated from hypersaline soils and emended description of Nocardiopsis salina Li et al. 2004. Int J Syst Evol Microbiol. 2006; 56(Pt 5):1089-1096. DOI: 10.1099/ijs.0.64033-0. View

19.

Saturnino R, Machado N, Lopes J, Nepomuceno J . Assessment of the mutagenic, recombinogenic, and carcinogenic potential of amphotericin B in somatic cells of Drosophila melanogaster. Drug Chem Toxicol. 2017; 41(1):9-15. DOI: 10.1080/01480545.2016.1188302. View

20.

Valour F, Senechal A, Dupieux C, Karsenty J, Lustig S, Breton P . Actinomycosis: etiology, clinical features, diagnosis, treatment, and management. Infect Drug Resist. 2014; 7:183-97. PMC: 4094581. DOI: 10.2147/IDR.S39601. View