Immunocytochemical Localization of Cathepsins B and H in Rat Liver

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1987 Jan 1

PMID 3325480

Citations 4

Authors

S Yokota

K Kato

Affiliations

Soon will be listed here.

Abstract

Light and electron microscopic localization of cathepsins B and H in rat liver was investigated by immunoenzyme and protein A-gold techniques. For light microscopy (LM), semi-thin sections of the Epon-embedded material were stained by the immunoenzyme technique after removal of epoxy resin. For electron microscopy (EM), ultra-thin sections of the Lowicryl K4M-embedded material were stained by the protein A-gold technique. By LM, reaction deposits for cathepsins B and H were present in the cytoplasmic granules of parenchymal cells and endothelial cells, and Kupffer cells. The sinus-lining cells and the parenchymal cells showed the similar staining intensity. By EM, gold particles were present exclusively in lysosomes of all the cell types cited above. The same results were obtained from quantitative analysis. In addition, Golgi complexes themselves were mostly negative but some small vesicles on the trans side of them were labeled for these proteinases. The results indicate that cathepsins B and H are present in the lysosomes of rat liver and that these enzymes seem to be transported by small vesicles from endoplasmic reticulum to lysosomes via tubuloreticular network of the trans Golgi region.

Citing Articles

Expression of cathepsin H in differentiating rat spermatids: immunoelectron microscopic study.

Haraguchi C, Ishido K, Kominami E, Yokota S Histochem Cell Biol. 2003; 120(1):63-71.

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Localization of acid phosphatase activity in collagen-secreting and collagen-resorbing fibroblasts.

Yajima T Histochemistry. 1988; 90(4):245-53.

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Immunoenzyme localization of cathepsins in the Golgi region of rat hepatocytes and renal tubule cells.

Yokota S, Nishimura Y, Kawabata T, Kato K Histochemistry. 1990; 94(6):629-35.

PMID: 2279958 DOI: 10.1007/BF00271990.

New sensitive light microscopical detection of colloidal gold on ultrathin sections by reflection contrast microscopy. Combination of reflection contrast and electron microscopy in post-embedding immunogold histochemistry.

Velde I, Prins F Histochemistry. 1990; 94(1):61-71.

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