Mapping the Alterations of Glutamate Using Glu-Weighted CEST MRI in a Rat Model of Fatigue

Overview

Journal Front Neurol

Specialty Neurology

Date 2020 Nov 30

PMID 33250853

Citations 1

Authors

Ruili Li

Zhuozhi Dai

Di Hu

Haiyan Zeng

Zeman Fang

Zerui Zhuang

Haiyun Xu

Qingjun Huang

Yilong Cui

Handi Zhang

Affiliations

Soon will be listed here.

Abstract

Glutamate dysregulation may play an important role in the pathophysiology of fatigue. Glutamate weighted chemical exchange saturation transfer (Glu-weighted CEST) MRI is a recently developed technology which enables measuring glutamate with high sensitivity and spatial resolution. The purpose of this study is to map the alternations of brain glutamate in a rat model of fatigue. Rats were subjected to 10 days fatigue loading procedure (fatigue group) or reared without any fatigue loading (control group). Spontaneous activities of rats in the fatigue group were recorded from 3 days before fatigue loading to 4 days after the end of fatigue loading. Glu-weighted CEST were performed following 10-day fatigue loading. Rats in the fatigue group exhibited significant reduced spontaneous activities after 10-day fatigue loading. The glutamate level in the whole brain increased significantly in the fatigue group compared to that in the control group. Further analysis of glutamate in the sub-regions of brain including the prefrontal cortex, hippocampus, and striatum revealed a trend of increment, although statistical significance was not reached. The increase of glutamate level in the brain may be a crucial process involved in the pathophysiology of fatigue.

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PMID: 37216423 PMC: 10252850. DOI: 10.1021/acschemneuro.3c00180.

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