Diaporthe Seed Decay of Soybean [ (L.) Merr.] Is Endemic in the United States, But New Fungi Are Involved

Diaporthe seed decay can compromise seed quality in soybean [ (L.) Merr.] in the warm and humid production areas of the United States during crop maturation. In the current study, 45 isolates of were recovered from seed sampled from soybean fields affected by -associated diseases in eight U.S. states in 2017. The isolates obtained belonged to 10 species of based on morphology and phylogenetic analyses of the internal transcribed spacer, partial translation elongation factor 1-α, and β-tubulin gene sequences. The associated species included , , , , , , , and three novel fungi, , , and . One isolate each of the 10 species was examined for pathogenicity on seed of cultivar Sava under controlled conditions. Seven days postinoculation, significant differences in the percentages of decayed seeds and seedling necrosis were observed among the isolates and the noninoculated control ( < 0.0001). While the isolates of , , and caused a significantly greater percentage of decayed seeds ( < 0.0001), the isolate of caused the greatest seedling necrosis ( < 0.0001). The observation of new fungi causing Diaporthe seed decay suggests the need for a more comprehensive survey in U.S. soybean producing areas since members of the genus appear to form a complex that causes seed decay.