Live Imaging of the Lung

Overview

Journal Curr Protoc Cytom

Publisher Wiley

Specialty Cell Biology

Date 2020 Nov 23

PMID 33226733

Citations 7

Authors

Tomasz Brzoska

Tomasz W Kaminski

Margaret F Bennewitz

Prithu Sundd

Affiliations

Soon will be listed here.

Abstract

Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion brought about by ventilation and cardiac contractions. A previous version of this Current Protocols in Cytometry article reported protocols for imaging ex vivo live lung slices and the intact mouse lung. Here, we update those protocols by adding new methodologies, new approaches for quantitative image analysis, and new areas of potential application. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Live imaging of lung slices Support Protocol 1: Staining lung sections with fluorescent antibodies Basic Protocol 2: Live imaging in the mouse lung Support Protocol 2: Intratracheal instillations Support Protocol 3: Intravascular instillations Support Protocol 4: Monitoring vital signs of the mouse during live lung imaging Support Protocol 5: Antibodies Support Protocol 6: Fluorescent reporter mice Basic Protocol 3: Quantification of neutrophil-platelet aggregation in pulmonary vasculature Basic Protocol 4: Quantification of platelet-dependent pulmonary thrombosis Basic Protocol 5: Quantification of pulmonary vascular permeability.

Citing Articles

Neutrophils and galectin-3 defend mice from lethal bacterial infection and humans from acute respiratory failure.

Das S, Kaminski T, Schlegel B, Bain W, Hu S, Patel A Nat Commun. 2024; 15(1):4724.

PMID: 38830855 PMC: 11148175. DOI: 10.1038/s41467-024-48796-y.

Lung microvascular occlusion by platelet-rich neutrophil-platelet aggregates promotes cigarette smoke-induced severe flu.

Kaminski T, Brzoska T, Li X, Vats R, Katoch O, Dubey R JCI Insight. 2023; 9(2).

PMID: 38060312 PMC: 10906226. DOI: 10.1172/jci.insight.167299.

Infection with Influenzavirus A in a murine model induces epithelial bronchial lesions and distinct waves of innate immune-cell recruitment.

Riviere F, Burger J, Lefevre F, Garnier A, Vigne C, Tournier J Front Immunol. 2023; 14:1241323.

PMID: 37649477 PMC: 10464834. DOI: 10.3389/fimmu.2023.1241323.

A Brief Overview of Neutrophils in Neurological Diseases.

Chakraborty S, Tabrizi Z, Bhatt N, Franciosa S, Bracko O Biomolecules. 2023; 13(5).

PMID: 37238612 PMC: 10216532. DOI: 10.3390/biom13050743.

Surrogate infection model predicts optimal alveolar macrophage number for clearance of Aspergillus fumigatus infections.

Saffer C, Timme S, Rudolph P, Figge M NPJ Syst Biol Appl. 2023; 9(1):12.

PMID: 37037824 PMC: 10086013. DOI: 10.1038/s41540-023-00272-x.

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