In Silico Identification of Effector Proteins from Generalist Herbivore Spodoptera Litura

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2020 Nov 23

PMID 33225897

Citations 6

Authors

Vinod Kumar Prajapati

Mahendra Varma

Jyothilakshmi Vadassery

Affiliations

Soon will be listed here.

Abstract

Background: The common cutworm, Spodoptera litura Fabricius is a leaf and fruit feeding generalist insect of the order Lepidoptera and a destructive agriculture pest. The broad host range of the herbivore is due to its ability to downregulate plant defense across different plants. The identity of Spodoptera litura released effectors that downregulate plant defense are largely unknown. The current study aims to identify genes encoding effector proteins from salivary glands of S. litura (Fab.).

Results: Head and salivary glands of Spodoptera litura were used for de-novo transcriptome analysis and effector prediction. Eight hundred ninety-nine proteins from the head and 330 from salivary gland were identified as secretory proteins. Eight hundred eight proteins from the head and 267 from salivary gland proteins were predicted to be potential effector proteins.

Conclusions: This study is the first report on identification of potential effectors from Spodoptera litura salivary glands.

Citing Articles

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References

Mithofer A, Boland W . Recognition of herbivory-associated molecular patterns. Plant Physiol. 2008; 146(3):825-31. PMC: 2259064. DOI: 10.1104/pp.107.113118. View

Zhao C, Escalante L, Chen H, Benatti T, Qu J, Chellapilla S . A massive expansion of effector genes underlies gall-formation in the wheat pest Mayetiola destructor. Curr Biol. 2015; 25(5):613-20. DOI: 10.1016/j.cub.2014.12.057. View

Schmelz E, Engelberth J, Alborn H, Tumlinson 3rd J, Teal P . Phytohormone-based activity mapping of insect herbivore-produced elicitors. Proc Natl Acad Sci U S A. 2009; 106(2):653-7. PMC: 2626758. DOI: 10.1073/pnas.0811861106. View

Kusnierczyk A, Winge P, Jorstad T, Troczynska J, Rossiter J, Bones A . Towards global understanding of plant defence against aphids--timing and dynamics of early Arabidopsis defence responses to cabbage aphid (Brevicoryne brassicae) attack. Plant Cell Environ. 2008; 31(8):1097-115. DOI: 10.1111/j.1365-3040.2008.01823.x. View

Zhang Y, Fan J, Sun J, Francis F, Chen J . Transcriptome analysis of the salivary glands of the grain aphid, Sitobion avenae. Sci Rep. 2017; 7(1):15911. PMC: 5698471. DOI: 10.1038/s41598-017-16092-z. View

Acevedo F, Rivera-Vega L, Chung S, Ray S, Felton G . Cues from chewing insects - the intersection of DAMPs, HAMPs, MAMPs and effectors. Curr Opin Plant Biol. 2015; 26:80-6. DOI: 10.1016/j.pbi.2015.05.029. View

Ji R, Ye W, Chen H, Zeng J, Li H, Yu H . A Salivary Endo-β-1,4-Glucanase Acts as an Effector That Enables the Brown Planthopper to Feed on Rice. Plant Physiol. 2017; 173(3):1920-1932. PMC: 5338667. DOI: 10.1104/pp.16.01493. View

Ahmad S, Veyrat N, Gordon-Weeks R, Zhang Y, Martin J, Smart L . Benzoxazinoid metabolites regulate innate immunity against aphids and fungi in maize. Plant Physiol. 2011; 157(1):317-27. PMC: 3165881. DOI: 10.1104/pp.111.180224. View

Fand B, Sul N, Bal S, Minhas P . Temperature Impacts the Development and Survival of Common Cutworm (Spodoptera litura): Simulation and Visualization of Potential Population Growth in India under Warmer Temperatures through Life Cycle Modelling and Spatial Mapping. PLoS One. 2015; 10(4):e0124682. PMC: 4415793. DOI: 10.1371/journal.pone.0124682. View

10.

Song F, Chen C, Wu S, Shao E, Li M, Guan X . Transcriptional profiling analysis of Spodoptera litura larvae challenged with Vip3Aa toxin and possible involvement of trypsin in the toxin activation. Sci Rep. 2016; 6:23861. PMC: 4812304. DOI: 10.1038/srep23861. View

11.

Zebelo S, Piorkowski J, Disi J, Fadamiro H . Secretions from the ventral eversible gland of Spodoptera exigua caterpillars activate defense-related genes and induce emission of volatile organic compounds in tomato, Solanum lycopersicum. BMC Plant Biol. 2014; 14:140. PMC: 4032488. DOI: 10.1186/1471-2229-14-140. View

12.

Bennett R, Wallsgrove R . Secondary metabolites in plant defence mechanisms. New Phytol. 2021; 127(4):617-633. DOI: 10.1111/j.1469-8137.1994.tb02968.x. View

13.

Francischetti I, Lopes A, Dias F, Pham V, Ribeiro J . An insight into the sialotranscriptome of the seed-feeding bug, Oncopeltus fasciatus. Insect Biochem Mol Biol. 2007; 37(9):903-10. PMC: 2904962. DOI: 10.1016/j.ibmb.2007.04.007. View

14.

Meena M, Prajapati R, Krishna D, Divakaran K, Pandey Y, Reichelt M . The Ca Channel CNGC19 Regulates Arabidopsis Defense Against Spodoptera Herbivory. Plant Cell. 2019; 31(7):1539-1562. PMC: 6635850. DOI: 10.1105/tpc.19.00057. View

15.

Vandermoten S, Harmel N, Mazzucchelli G, De Pauw E, Haubruge E, Francis F . Comparative analyses of salivary proteins from three aphid species. Insect Mol Biol. 2014; 23(1):67-77. DOI: 10.1111/imb.12061. View

16.

Saunders D, Win J, Cano L, Szabo L, Kamoun S, Raffaele S . Using hierarchical clustering of secreted protein families to classify and rank candidate effectors of rust fungi. PLoS One. 2012; 7(1):e29847. PMC: 3253089. DOI: 10.1371/journal.pone.0029847. View

17.

Emanuelsson O, Nielsen H, Brunak S, von Heijne G . Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol. 2000; 300(4):1005-16. DOI: 10.1006/jmbi.2000.3903. View

18.

Consales F, Schweizer F, Erb M, Gouhier-Darimont C, Bodenhausen N, Bruessow F . Insect oral secretions suppress wound-induced responses in Arabidopsis. J Exp Bot. 2011; 63(2):727-37. PMC: 3254683. DOI: 10.1093/jxb/err308. View

19.

Calvo E, Mans B, Andersen J, Ribeiro J . Function and evolution of a mosquito salivary protein family. J Biol Chem. 2005; 281(4):1935-42. DOI: 10.1074/jbc.M510359200. View

20.

Moller S, Croning M, Apweiler R . Evaluation of methods for the prediction of membrane spanning regions. Bioinformatics. 2001; 17(7):646-53. DOI: 10.1093/bioinformatics/17.7.646. View