Baicalin Liposome Alleviates Lipopolysaccharide-Induced Acute Lung Injury in Mice Via Inhibiting TLR4/JNK/ERK/NF-B Pathway

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2020 Nov 23

PMID 33223957

Citations 16

Authors

Yu Long

Yan Xiang

Songyu Liu

Yulu Zhang

Jinyan Wan

Qiyue Yang

Mingquan Cui

Zhimin Ci

Nan Li

Wei Peng

Affiliations

Soon will be listed here.

Abstract

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are challenging diseases with the high mortality in a clinical setting. Baicalin (BA) is the main effective constituent isolated from the Chinese medical herb Georgi, and studies have proved that it has a protective effect on ALI induced by lipopolysaccharide (LPS) due to the anti-inflammatory efficacy. However, BA has low solubility which may limit its clinical application. Hence, we prepared a novel drug delivery system-Baicalin liposome (BA-LP) in previous research-which can improve some physical properties of BA. Therefore, we aimed to explore the effect of BA-LP on ALI mice induced by LPS. In pharmacokinetics study, the values of and AUC in the BA-LP group were significantly higher than that of the BA group in normal mice, indicating that BA-LP could prolong the duration time of BA. The BA-LP group also showed a higher concentration in lung tissues than the BA group. Pharmacodynamics studies showed that BA-LP had a better effect than the BA group at the same dosage on reducing the W/D ratio, alleviating the lung injury score, and decreasing the proinflammatory factors (TNF-, IL-1) and total proteins in bronchoalveolar lavage fluids (BALF). In addition, the therapeutic effects of BA-LP showed a dose-dependent manner. Western blot analysis indicated that the anti-inflammatory action of BA could be attributed to the inhibition of the TLR4-NFBp65 and JNK-ERK signaling pathways. These results suggest that BA-LP could be a valuable therapeutic candidate in the treatment of ALI.

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References

Li N, Feng L, Tan Y, Xiang Y, Zhang R, Yang M . Preparation, Characterization, Pharmacokinetics and Biodistribution of Baicalin-Loaded Liposome on Cerebral Ischemia-Reperfusion after i.v. Administration in Rats. Molecules. 2018; 23(7). PMC: 6100585. DOI: 10.3390/molecules23071747. View

Lei J, Wei Y, Song P, Li Y, Zhang T, Feng Q . Cordycepin inhibits LPS-induced acute lung injury by inhibiting inflammation and oxidative stress. Eur J Pharmacol. 2017; 818:110-114. DOI: 10.1016/j.ejphar.2017.10.029. View

Goodman R, Pugin J, Lee J, Matthay M . Cytokine-mediated inflammation in acute lung injury. Cytokine Growth Factor Rev. 2003; 14(6):523-35. DOI: 10.1016/s1359-6101(03)00059-5. View

Welty-Wolf K, Carraway M, Ortel T, Piantadosi C . Coagulation and inflammation in acute lung injury. Thromb Haemost. 2002; 88(1):17-25. View

Fu S, Lu W, Yu W, Hu J . Protective effect of extract on lipopolysaccharide-induced acute lung injury in mice. Biosci Rep. 2019; 39(6). PMC: 6591570. DOI: 10.1042/BSR20190789. View

Roach S, Lee S, Schorey J . Differential activation of the transcription factor cyclic AMP response element binding protein (CREB) in macrophages following infection with pathogenic and nonpathogenic mycobacteria and role for CREB in tumor necrosis factor alpha production. Infect Immun. 2004; 73(1):514-22. PMC: 538945. DOI: 10.1128/IAI.73.1.514-522.2005. View

Hu X, Tian Y, Qu S, Cao Y, Li S, Zhang W . Protective effect of TM6 on LPS-induced acute lung injury in mice. Sci Rep. 2017; 7(1):572. PMC: 5428560. DOI: 10.1038/s41598-017-00551-8. View

Wright S, Ramos R, Tobias P, Ulevitch R, Mathison J . CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990; 249(4975):1431-3. DOI: 10.1126/science.1698311. View

Moine P, McIntyre R, Schwartz M, Kaneko D, Shenkar R, Le Tulzo Y . NF-kappaB regulatory mechanisms in alveolar macrophages from patients with acute respiratory distress syndrome. Shock. 2000; 13(2):85-91. DOI: 10.1097/00024382-200013020-00001. View

10.

Gando S, Kameue T, Matsuda N, Sawamura A, Hayakawa M, Kato H . Systemic inflammation and disseminated intravascular coagulation in early stage of ALI and ARDS: role of neutrophil and endothelial activation. Inflammation. 2005; 28(4):237-44. DOI: 10.1023/b:ifla.0000049049.81688.fe. View

11.

Wang W, Weng J, Yu L, Huang Q, Jiang Y, Guo X . Role of TLR4-p38 MAPK-Hsp27 signal pathway in LPS-induced pulmonary epithelial hyperpermeability. BMC Pulm Med. 2018; 18(1):178. PMC: 6258407. DOI: 10.1186/s12890-018-0735-0. View

12.

Wu Y, Wang F, Fan L, Zhang W, Wang T, Du Y . Baicalin alleviates atherosclerosis by relieving oxidative stress and inflammatory responses via inactivating the NF-κB and p38 MAPK signaling pathways. Biomed Pharmacother. 2018; 97:1673-1679. DOI: 10.1016/j.biopha.2017.12.024. View

13.

Reutershan J, Basit A, Galkina E, Ley K . Sequential recruitment of neutrophils into lung and bronchoalveolar lavage fluid in LPS-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2005; 289(5):L807-15. DOI: 10.1152/ajplung.00477.2004. View

14.

Tao L, Cao F, Xu G, Xie H, Zhang M, Zhang C . Mogroside IIIE Attenuates LPS-Induced Acute Lung Injury in Mice Partly Through Regulation of the TLR4/MAPK/NF-κB Axis via AMPK Activation. Phytother Res. 2017; 31(7):1097-1106. DOI: 10.1002/ptr.5833. View

15.

Xu C, Chen G, Yang W, Xu Y, Xu Y, Huang X . Hyaluronan ameliorates LPS-induced acute lung injury in mice via Toll-like receptor (TLR) 4-dependent signaling pathways. Int Immunopharmacol. 2015; 28(2):1050-8. DOI: 10.1016/j.intimp.2015.08.021. View

16.

Wang B, Gong X, Wan J, Zhang L, Zhang Z, Li H . Resolvin D1 protects mice from LPS-induced acute lung injury. Pulm Pharmacol Ther. 2011; 24(4):434-41. DOI: 10.1016/j.pupt.2011.04.001. View

17.

Yang Y, Ding Z, Wang Y, Zhong R, Feng Y, Xia T . Systems pharmacology reveals the mechanism of activity of Physalis alkekengi L. var. franchetii against lipopolysaccharide-induced acute lung injury. J Cell Mol Med. 2020; 24(9):5039-5056. PMC: 7205831. DOI: 10.1111/jcmm.15126. View

18.

Fan E, Brodie D, Slutsky A . Acute Respiratory Distress Syndrome: Advances in Diagnosis and Treatment. JAMA. 2018; 319(7):698-710. DOI: 10.1001/jama.2017.21907. View

19.

Tianzhu Z, Shumin W . Esculin Inhibits the Inflammation of LPS-Induced Acute Lung Injury in Mice Via Regulation of TLR/NF-κB Pathways. Inflammation. 2015; 38(4):1529-36. DOI: 10.1007/s10753-015-0127-z. View

20.

Meng X, Hu L, Li W . Baicalin ameliorates lipopolysaccharide-induced acute lung injury in mice by suppressing oxidative stress and inflammation via the activation of the Nrf2-mediated HO-1 signaling pathway. Naunyn Schmiedebergs Arch Pharmacol. 2019; 392(11):1421-1433. DOI: 10.1007/s00210-019-01680-9. View