Nucleotide Sequence of the Klebsiella Pneumoniae NifD Gene and Predicted Amino Acid Sequence of the Alpha-subunit of Nitrogenase MoFe Protein

Overview

Journal Biochem J

Specialty Biochemistry

Date 1987 Oct 15

PMID 3322262

Citations 5

Authors

I Ioannidis

M Buck

Affiliations

Soon will be listed here.

Abstract

The nucleotide sequence of the Klebsiella pneumoniae nifD gene is presented and together with the accompanying paper [Holland, Zilberstein, Zamir & Sussman (1987) Biochem. J. 247, 277-285] completes the sequence of the nifHDK genes encoding the nitrogenase polypeptides. The K. pneumoniae nifD gene encodes the 483-amino acid-residue nitrogenase alpha-subunit polypeptide of Mr 54156. The alpha-subunit has five strongly conserved cysteine residues at positions 63, 89, 155, 184 and 275, some occurring in a region showing both primary sequence and potential structural homology to the K. pneumoniae nitrogenase beta-subunit. A comparison with six other alpha-subunit amino acid sequences has been made, which indicates a number of potentially important domains within alpha-subunits.

Citing Articles

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Nucleotide and deduced amino acid sequences of the Klebsiella pneumoniae nifK gene coding for the beta-subunit of nitrogenase MoFe protein.

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Structure-function relationships in the alpha subunit of Klebsiella pneumoniae nitrogenase MoFe protein from analysis of nifD mutants.

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