Anti-inflammatory Effects of Astaxanthin Against Fungal Keratitis

Overview

Journal Int J Ophthalmol

Specialty Ophthalmology

Date 2020 Nov 20

PMID 33214996

Authors

Yu Huan

Xu-Dong Peng

Jing Lin

Ying-Xue Zhang

Lu Zhan

Han Gao

Gui-Qiu Zhao

Affiliations

Soon will be listed here.

Abstract

Aim: To characterize effect of astaxanthin (ASX) in () induced keratitis in mouse model.

Methods: , fungal keratitis mouse model was established in C57BL/6 mice using , followed by ASX or dimethyl sulfoxide (DMSO) treatment. Clinical responses were evaluated by clinical score and myeloperoxidase (MPO) assay. Inflammatory cytokines were assessed by reverse-transcription polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, and enzyme-linked immuno sorbent assay (ELISA).

Results: In animal model, ASX improved corneal transparency and clinical response, suppressed the expression of inflammatory cytokine like IL-1β, TNF-α, and HMGB-1. Neutrophil levels have been shown to decrease in ASX-treated cornea by immunofluorescence and MPO. TLR2 and TLR4 levels were lower in ASX-treated group than DMSO-treated.

Conclusion: ASX can suppress inflammatory response and reduce inflammatory cytokine production in mice model with keratitis.

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