is Important for Mammalian Neural Tube Closure Via Its Role in Canonical and Non-canonical WNT Signaling

Overview

Journal Development

Specialty Biology

Date 2020 Nov 20

PMID 33214242

Citations 8

Authors

Heather Mary Brown

Stephen A Murray

Hope Northrup

Kit Sing Au

Lee A Niswander

Affiliations

Soon will be listed here.

Abstract

Disruptions in neural tube (NT) closure result in neural tube defects (NTDs). To understand the molecular processes required for mammalian NT closure, we investigated the role of , a sorting nexin gene. mutant mouse embryos display a fully-penetrant cranial NTD. , we observed decreased canonical WNT target gene expression in the cranial neural epithelium of the embryos and a defect in convergent extension of the neural epithelium. cells show decreased WNT secretion, and live cell imaging reveals aberrant recycling of the WNT ligand-binding protein WLS and mis-trafficking to the lysosome for degradation. The importance of SNX3 in WNT signaling regulation is demonstrated by rescue of NT closure in embryos with a WNT agonist. The potential for SNX3 to function in human neurulation is revealed by a point mutation identified in an NTD-affected individual that results in functionally impaired SNX3 that does not colocalize with WLS and the degradation of WLS in the lysosome. These data indicate that is crucial for NT closure via its role in recycling WLS in order to control levels of WNT signaling.

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