Diagnostic Value of Urinary Exosomal MiR-23b-3p, MiR-30a-5p, and MiR-151-3p in Children with Primary Nephrotic Syndrome

Overview

Journal Transl Androl Urol

Date 2020 Nov 19

PMID 33209688

Citations 5

Authors

Dan Feng

Boying Wu

Yajiao Pang

Affiliations

Soon will be listed here.

Abstract

Background: Primary nephrotic syndrome (NS) is a common disease of the urinary system with an unclear pathogenesis. We aimed to detect the levels of urinary exosomal miR-23b-3p, miR-30a-5p, and miR-151-3p in children with primary NS, and to explore their diagnostic value for NS.

Methods: A total of 115 patients with NS who were admitted to the hospital from June 2017 to June 2019 were selected as the observation group. According to the disease progression, they were divided into an active group (acute active phase, n=68) and remission group (remission phase, n=47). In all, 50 healthy children were selected as the control group. Levels of urinary exosomal miR-23b-3p, miR-30a-5p, and miR-151-3p of each group in different periods were detected.

Results: The 24-h urine protein, serum albumin (ALB), and serum total cholesterol (TC) levels were significantly higher in the observation group than in the control group (P<0.05), while those in the active group were significantly higher than those in the remission group (P<0.05). The levels of miR-23b-3p and miR-30a-5p were significantly higher in the observation group than in the control group, and significantly higher in the active group than in the remission group (P<0.05). No miR-151-3p was detected in the urinary exosomes of the two groups. After treatment, levels of exosomal miR-23b-3p and miR-30a-5p in the two groups both decreased significantly (P<0.05). Results of receiver operating curve (ROC) curve analysis showed that urinary exosomal miR-23b-3p and miR-30a-5p can be used to identify children with NS and healthy children. The area under the ROC curve (AUC) was 0.711 for miR-23b-3p and 0.844 for miR-30a-5p.

Conclusions: The levels of miR-23b-3p and miR-30a-5p in urinary exosomes of children with NS were significantly higher than those in healthy children, and decreased significantly after treatment, indicating that miR-23b-3p and miR-30a-5p in urinary exosomes are potential indicators for diagnosing the progression of NS and monitoring the treatment effect.

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References

McCloskey O, Maxwell A . Diagnosis and management of nephrotic syndrome. Practitioner. 2017; 261(1801):11-5. View

Luo Y, Wang C, Chen X, Zhong T, Cai X, Chen S . Increased serum and urinary microRNAs in children with idiopathic nephrotic syndrome. Clin Chem. 2013; 59(4):658-66. DOI: 10.1373/clinchem.2012.195297. View

Xia X, Wang Y, Huang Y, Zhang H, Lu H, Zheng J . Exosomal miRNAs in central nervous system diseases: biomarkers, pathological mediators, protective factors and therapeutic agents. Prog Neurobiol. 2019; 183:101694. PMC: 7323939. DOI: 10.1016/j.pneurobio.2019.101694. View

Tang Y, Yan W, Sun L, Huang J, Qian W, Ding Y . Prediction of intravenous immunoglobulin resistance in Kawasaki disease in an East China population. Clin Rheumatol. 2016; 35(11):2771-2776. DOI: 10.1007/s10067-016-3370-2. View

Ho J, Ng K, Rosen S, Dostal A, Gregory R, Kreidberg J . Podocyte-specific loss of functional microRNAs leads to rapid glomerular and tubular injury. J Am Soc Nephrol. 2008; 19(11):2069-75. PMC: 2573018. DOI: 10.1681/ASN.2008020162. View

Wang C, Greenbaum L . Nephrotic Syndrome. Pediatr Clin North Am. 2018; 66(1):73-85. DOI: 10.1016/j.pcl.2018.08.006. View

Liu Y, Liu B, Liu Y, Chen S, Yang J, Liu J . MicroRNA expression profile by next-generation sequencing in a novel rat model of contrast-induced acute kidney injury. Ann Transl Med. 2019; 7(8):178. PMC: 6526253. DOI: 10.21037/atm.2019.04.44. View

Lorenzen J, Kielstein J, Hafer C, Gupta S, Kumpers P, Faulhaber-Walter R . Circulating miR-210 predicts survival in critically ill patients with acute kidney injury. Clin J Am Soc Nephrol. 2011; 6(7):1540-6. DOI: 10.2215/CJN.00430111. View

Huang Z, Zhang Y, Zhou J, Zhang Y . Urinary Exosomal miR-193a Can Be a Potential Biomarker for the Diagnosis of Primary Focal Segmental Glomerulosclerosis in Children. Biomed Res Int. 2017; 2017:7298160. PMC: 5303601. DOI: 10.1155/2017/7298160. View

10.

Kapogiannis D . Exosome Biomarkers Revolutionize Preclinical Diagnosis of Neurodegenerative Diseases and Assessment of Treatment Responses in Clinical Trials. Adv Exp Med Biol. 2020; 1195:149. DOI: 10.1007/978-3-030-32633-3_19. View

11.

Najafian B, Mauer M, Hopkin R, Svarstad E . Renal complications of Fabry disease in children. Pediatr Nephrol. 2012; 28(5):679-87. PMC: 3811930. DOI: 10.1007/s00467-012-2222-9. View

12.

Kimura H, Kawasaki H, Taira K . Mouse microRNA-23b regulates expression of Hes1 gene in P19 cells. Nucleic Acids Symp Ser (Oxf). 2006; (48):213-4. DOI: 10.1093/nass/48.1.213. View

13.

Agrawal R, Tran U, Wessely O . The miR-30 miRNA family regulates Xenopus pronephros development and targets the transcription factor Xlim1/Lhx1. Development. 2009; 136(23):3927-36. PMC: 2778741. DOI: 10.1242/dev.037432. View

14.

Barkai L, Soos A, Vamosi I . [The effect of captopril therapy on the degree of microalbuminuria in diabetic children and adolescents with incipient nephropathy]. Orv Hetil. 1996; 137(46):2565-8. View

15.

Souilmi F, Houssaini T, Alaoui H, Harmouch T, Atmani S, Hida M . Indications and results of renal biopsy in children: A single-center experience from Morocco. Saudi J Kidney Dis Transpl. 2015; 26(4):810-5. DOI: 10.4103/1319-2442.160225. View

16.

Kodner C . Diagnosis and Management of Nephrotic Syndrome in Adults. Am Fam Physician. 2016; 93(6):479-85. View

17.

Zhang J, Li S, Li L, Li M, Guo C, Yao J . Exosome and exosomal microRNA: trafficking, sorting, and function. Genomics Proteomics Bioinformatics. 2015; 13(1):17-24. PMC: 4411500. DOI: 10.1016/j.gpb.2015.02.001. View