Rapid Identification of KL49 Associated with Clinical Mortality

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2020 Nov 19

PMID 33209042

Citations 2

Authors

Qiuyang Deng

Jinyong Zhang

Min Zhang

Zhou Liu

Yuxin Zhong

Shiyi Liu

Ruiqin Cui

Yun Shi

Hao Zeng

Xiyao Yang

Chuchu Lin

Yutian Luo

Huaisheng Chen

Weiyuan Wu

Jinsong Wu

Tianle Zhang

Yuemei Lu

Xueyan Liu

Quanming Zou

Wei Huang

Affiliations

Soon will be listed here.

Abstract

Objective: We aimed to establish a tool for rapid identification of KL49 .

Methods: Based on the capsular polysaccharide (CPS) synthesis genes database, we investigated the distribution of K locus type 49 (KL49) genes in other KL types and established a rapid identification method for KL49. We collected 61 clinical carbapenem-resistant (CRAB) strains, identified KL49 by detection, and used whole genome sequencing (WGS) for verification. A mouse pneumonia model was used to confirm the hypervirulence phenotype. We tested the presence of gene in 165 CRAB strains from three provinces in China and evaluated the correlation of carrying CRAB infection with mortality.

Results: The gene is the CPS synthesis gene found only in KL49. We screened out nine WGS-validated KL49 strains from 61 CRAB clinical strains using polymerase chain reaction (PCR) to detect the gene. The survival rates of KL49 strains were significantly lower than nonKL49 strains in a mouse pneumonia model. The survival rates of LAC-4 knockout strain decreased significantly. Analysis of phylogenetics showed the worldwide spread of KL49 . Infection of carrying CRAB is an independent risk for mortality (OR, 10.76; 95%CI: 3.08-37.55; <0.001).

Conclusion: The hypervirulence phenotype of KL49 CRAB and the association with mortality highlight the urgent need for implementing control measures. The rapid identification assay has the potential to facilitate early medical intervention and worldwide surveillance.

Citing Articles

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An update to the database for capsular polysaccharide locus typing extends the extensive and diverse repertoire of genes found at and outside the K locus.

Cahill S, Hall R, Kenyon J Microb Genom. 2022; 8(10).

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