Molecular Epidemiology of Isolates in Iran Using Spoligotyping

Overview

Journal New Microbes New Infect

Publisher Elsevier

Specialty Infectious Diseases

Date 2020 Nov 18

PMID 33204430

Citations 4

Authors

R Ramazanzadeh

P Shakib

S Rouhi

B Mohammadi

P Mohajeri

S Borji

Affiliations

Soon will be listed here.

Abstract

Spoligotyping can help assess the transmission of strains. We aimed to study the genotyping of isolated from patients with tuberculosis from the west of Iran by spoligotyping. Forty-seven isolates were collected from the west of Iran. All samples were cultured on Löwenstein-Jensen medium incubated at 37°C for 8 weeks. Bacterial isolates were identified as using standard biochemical tests. Drug resistance patterns of to rifampicin and isoniazid were determined, and multidrug-resistant (MDR) strains were isolated. After DNA extraction, spoligotyping was performed. We found new spoligotypes 4162 and 4163, which correlated with atypical lineage. Atypical and unknown lineages also had correlations with the MDR tuberculosis rate (4%). The most prevalent spoligointernational types were orphan (34%), 2669 (23.4%) and 127 (14.8%) types. The most prevalent clades were Ural-2 (NEW-1) (25.53%) and atypical (23.40%) lineages. The predominant clade was Ural-2 (NEW-1) and an atypical lineage restricted to Iran. The rate of MDR was low. Knowledge of the circulating isolates in the west of Iran will help implement control programmes, so knowledge of the dynamic transmission of local isolates is crucial.

Citing Articles

Antimicrobial resistance profile and prevalence of complex in Western Iran using spoligotyping method.

Borji S, Kooti S, Ramazanzadeh R, Kadivarian S, Atashi S, Mohajeri P J Clin Tuberc Other Mycobact Dis. 2024; 36:100467.

PMID: 39184015 PMC: 11342116. DOI: 10.1016/j.jctube.2024.100467.

Single-tube protocol for culture-independent spoligotyping of based on MeltArray.

Xia Z, Su B, Tu C, Sun S, Tan Y, Xu Y J Clin Microbiol. 2023; 62(1):e0118323.

PMID: 38112521 PMC: 10793361. DOI: 10.1128/jcm.01183-23.

Tuberculosis in Poland: Epidemiological and Molecular Analysis during the COVID-19 Pandemic.

Borkowska-Tatar D, Zabost A, Kozinska M, Augustynowicz-Kopec E Diagnostics (Basel). 2022; 12(8).

PMID: 36010233 PMC: 9406582. DOI: 10.3390/diagnostics12081883.

Determination of genetic diversity of multidrug-resistant strains in Turkey using 15 locus MIRU-VNTR and spoligotyping methods.

Gurer Giray B, Aslanturk A, Simsek H, Ozgur D, Kilic S, Aslan G Pathog Glob Health. 2022; 117(1):85-91.

PMID: 35642888 PMC: 9848327. DOI: 10.1080/20477724.2022.2084807.

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