Processing of a Wheat Light-harvesting Chlorophyll A/b Protein Precursor by a Soluble Enzyme from Higher Plant Chloroplasts

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1987 Dec 1

PMID 3320051

Citations 20

Authors

G K Lamppa

M S Abad

Affiliations

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Abstract

A processing activity has been identified in higher plant chloroplasts that cleaves the precursor of the light-harvesting chlorophyll a/b-binding protein (LHCP). A wheat LHCP gene previously characterized (Lamppa, G.K., G. Morelli, and N.-H. Chua, 1985. Mol. Cell Biol. 5:1370-1378) was used to synthesize RNA and subsequently the labeled precursor polypeptide in vitro. Incubation of the LHCP precursors with a soluble extract from lysed chloroplasts, after removal of the thylakoids and membrane vesicles, resulted in the release of a single 25-kD peptide. In contrast, when the LHCP precursors were used in an import reaction with intact pea or wheat chloroplasts, two forms (25 and 26 kD) of mature LHCP were found. The peptide released by the processing activity in the organelle-free assay comigrated with the lower molecular mass form of mature LHCP produced during import. Properties of the processing activity suggest that it is an endopeptidase. Chloroplasts from both pea and wheat, two divergent higher plants, contain the processing enzyme, suggesting its physiological importance in LHCP assembly into the thylakoids. We discuss the implications of LHCP precursor processing by a soluble enzyme that may be in the stromal compartment.

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References

Grossman A, Bartlett S, Schmidt G, Mullet J, Chua N . Optimal conditions for post-translational uptake of proteins by isolated chloroplasts. In vitro synthesis and transport of plastocyanin, ferredoxin-NADP+ oxidoreductase, and fructose-1,6-bisphosphatase. J Biol Chem. 1982; 257(3):1558-63. View

Smeekens S, Bauerle C, Hageman J, Keegstra K, Weisbeek P . The role of the transit peptide in the routing of precursors toward different chloroplast compartments. Cell. 1986; 46(3):365-75. DOI: 10.1016/0092-8674(86)90657-4. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Staehelin L, Arntzen C . Regulation of chloroplast membrane function: protein phosphorylation changes the spatial organization of membrane components. J Cell Biol. 1983; 97(5 Pt 1):1327-37. PMC: 2112674. DOI: 10.1083/jcb.97.5.1327. View

Lamppa G, Nagy F, Chua N . Light-regulated and organ-specific expression of a wheat Cab gene in transgenic tobacco. Nature. 1985; 316(6030):750-2. DOI: 10.1038/316750a0. View

Leutwiler L, Meyerowitz E, Tobin E . Structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in Arabidopsis thaliana. Nucleic Acids Res. 1986; 14(10):4051-64. PMC: 339845. DOI: 10.1093/nar/14.10.4051. View

Cline K, Andrews J, Mersey B, Newcomb E, Keegstra K . Separation and characterization of inner and outer envelope membranes of pea chloroplasts. Proc Natl Acad Sci U S A. 1981; 78(6):3595-9. PMC: 319617. DOI: 10.1073/pnas.78.6.3595. View

Bohni P, Daum G, Schatz G . Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in cleavage of mitochondrial precursor polypeptides. J Biol Chem. 1983; 258(8):4937-43. View

Schmidt G, Bartlett S, Grossman A, Cashmore A, Chua N . Biosynthetic pathways of two polypeptide subunits of the light-harvesting chlorophyll a/b protein complex. J Cell Biol. 1981; 91(2 Pt 1):468-78. PMC: 2111963. DOI: 10.1083/jcb.91.2.468. View

10.

Schmidt G, Devillers-Thiery A, Desruisseaux H, Blobel G, Chua N . NH2-terminal amino acid sequences of precursor and mature forms of the ribulose-1,5-bisphosphate carboxylase small subunit from Chlamydomonas reinhardtii. J Cell Biol. 1979; 83(3):615-22. PMC: 2110513. DOI: 10.1083/jcb.83.3.615. View

11.

Cashmore A . Structure and expression of a pea nuclear gene encoding a chlorophyll a/b-binding polypeptide. Proc Natl Acad Sci U S A. 1984; 81(10):2960-4. PMC: 345200. DOI: 10.1073/pnas.81.10.2960. View

12.

Lamppa G, Bendich A . Changes in Chloroplast DNA Levels during Development of Pea (Pisum sativum). Plant Physiol. 1979; 64(1):126-30. PMC: 543038. DOI: 10.1104/pp.64.1.126. View

13.

Schmidt R, Myers A, Gillham N, Boynton J . Chloroplast ribosomal proteins of Chlamydomonas synthesized in the cytoplasm are made as precursors. J Cell Biol. 1984; 98(6):2011-8. PMC: 2113070. DOI: 10.1083/jcb.98.6.2011. View

14.

Mullet J . The amino acid sequence of the polypeptide segment which regulates membrane adhesion (grana stacking) in chloroplasts. J Biol Chem. 1983; 258(16):9941-8. View

15.

Darr S, Somerville S, Arntzen C . Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II. J Cell Biol. 1986; 103(3):733-40. PMC: 2114296. DOI: 10.1083/jcb.103.3.733. View

16.

Schmidt G, Mishkind M . The transport of proteins into chloroplasts. Annu Rev Biochem. 1986; 55:879-912. DOI: 10.1146/annurev.bi.55.070186.004311. View

17.

Schmidt R, Gillham N, Boynton J . Processing of the precursor to a chloroplast ribosomal protein made in the cytosol occurs in two steps, one of which depends on a protein made in the chloroplast. Mol Cell Biol. 1985; 5(5):1093-9. PMC: 366826. DOI: 10.1128/mcb.5.5.1093-1099.1985. View

18.

Lamppa G, Morelli G, Chua N . Structure and developmental regulation of a wheat gene encoding the major chlorophyll a/b-binding polypeptide. Mol Cell Biol. 1985; 5(6):1370-8. PMC: 366866. DOI: 10.1128/mcb.5.6.1370-1378.1985. View

19.

Sheen J, Bogorad L . Differential expression of six light-harvesting chlorophyll a/b binding protein genes in maize leaf cell types. Proc Natl Acad Sci U S A. 1986; 83(20):7811-5. PMC: 386812. DOI: 10.1073/pnas.83.20.7811. View

20.

Chua N, Schmidt G . Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc Natl Acad Sci U S A. 1978; 75(12):6110-4. PMC: 393128. DOI: 10.1073/pnas.75.12.6110. View