Differential Expression of Genes Involved in Saikosaponin Biosynthesis Between DC. and Willd

Overview

Journal Front Genet

Date 2020 Nov 16

PMID 33193712

Citations 8

Authors

Ma Yu

Hua Chen

Shi-Hang Liu

Yu-Chan Li

Chun Sui

Da-Bin Hou

Jian-He Wei

Affiliations

Soon will be listed here.

Abstract

Radix Bupleuri (roots of spp.) is an important medicinal herb. Triterpenoid saponins of saikosaponins generally constitute the main class of secondary metabolites of plants in the genus. However, the molecular regulatory mechanism underlying their biosynthesis remains elusive. In this study, we observed significantly different saikosaponin biosynthesis between and at the seedling stage. The sequential and expression characterization of 232 genes in the triterpenoid saponin biosynthetic pathway, which includes the mevalonate (MVA) pathway and methylerythritol phosphate (MEP) pathway, between and was also investigated. Sixty of these genes may be involved in saikosaponin biosynthesis. Manipulation of these genes, especially those of the β-AS, P450, and UGT families, may improve saikosaponin production.

Citing Articles

Broadly Targeted Metabolomics Analysis of Differential Metabolites Between DC. and Willd.

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Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in DC.

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References

Liang Z, Oh K, Wang Y, Yi T, Chen H, Zhao Z . Cell type-specific qualitative and quantitative analysis of saikosaponins in three Bupleurum species using laser microdissection and liquid chromatography-quadrupole/time of flight-mass spectrometry. J Pharm Biomed Anal. 2014; 97:157-65. DOI: 10.1016/j.jpba.2014.04.033. View

Xue L, He Z, Bi X, Xu W, Wei T, Wu S . Transcriptomic profiling reveals MEP pathway contributing to ginsenoside biosynthesis in Panax ginseng. BMC Genomics. 2019; 20(1):383. PMC: 6524269. DOI: 10.1186/s12864-019-5718-x. View

Sui C, Chen M, Xu J, Wei J, Jin Y, Xu Y . Comparison of root transcriptomes and expressions of genes involved in main medicinal secondary metabolites from Bupleurum chinense and Bupleurum scorzonerifolium, the two Chinese official Radix bupleuri source species. Physiol Plant. 2014; 153(2):230-42. DOI: 10.1111/ppl.12254. View

Lin T, Chiou C, Chiou S . Putative genes involved in saikosaponin biosynthesis in Bupleurum species. Int J Mol Sci. 2013; 14(6):12806-26. PMC: 3709813. DOI: 10.3390/ijms140612806. View

Zhang Y, Zhou Z, Xia P, Liang Z, Liu S, Liu Z . [Expression of key enzyme genes and content of saikosaponin in saikosaponin biosynthesis under drought stress in Bupleurum chinense]. Zhongguo Zhong Yao Za Zhi. 2017; 41(4):643-647. DOI: 10.4268/cjcmm20160416. View

Anders S, Huber W . Differential expression analysis for sequence count data. Genome Biol. 2010; 11(10):R106. PMC: 3218662. DOI: 10.1186/gb-2010-11-10-r106. View

Hamberger B, Bak S . Plant P450s as versatile drivers for evolution of species-specific chemical diversity. Philos Trans R Soc Lond B Biol Sci. 2013; 368(1612):20120426. PMC: 3538417. DOI: 10.1098/rstb.2012.0426. View

Ikegami F, Sumino M, Fujii Y, Akiba T, Satoh T . Pharmacology and toxicology of Bupleurum root-containing Kampo medicines in clinical use. Hum Exp Toxicol. 2006; 25(8):481-94. DOI: 10.1191/0960327106het654oa. View

Ohnishi T, Szatmari A, Watanabe B, Fujita S, Bancos S, Koncz C . C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis. Plant Cell. 2006; 18(11):3275-88. PMC: 1693957. DOI: 10.1105/tpc.106.045443. View

10.

Wang T, Wang H, Cai D, Gao Y, Zhang H, Wang Y . Comprehensive profiling of rhizome-associated alternative splicing and alternative polyadenylation in moso bamboo (Phyllostachys edulis). Plant J. 2017; 91(4):684-699. DOI: 10.1111/tpj.13597. View

11.

Yang L, Jin Y, Huang W, Sun Q, Liu F, Huang X . Full-length transcriptome sequences of ephemeral plant Arabidopsis pumila provides insight into gene expression dynamics during continuous salt stress. BMC Genomics. 2018; 19(1):717. PMC: 6161380. DOI: 10.1186/s12864-018-5106-y. View

12.

Tan L, Cai X, Hu Z, Ni X . Localization and dynamic change of saikosaponin in root of Bupleurum chinense. J Integr Plant Biol. 2008; 50(8):951-7. DOI: 10.1111/j.1744-7909.2008.00668.x. View

13.

Hillier S, Lathe R . Terpenes, hormones and life: isoprene rule revisited. J Endocrinol. 2019; 242(2):R9-R22. DOI: 10.1530/JOE-19-0084. View

14.

Guo Y, Matsumoto T, Kikuchi Y, Ikejima T, Wang B, Yamada H . Effects of a pectic polysaccharide from a medicinal herb, the roots of Bupleurum falcatum L. on interleukin 6 production of murine B cells and B cell lines. Immunopharmacology. 2000; 49(3):307-16. DOI: 10.1016/s0162-3109(00)00245-9. View

15.

Pistelli L, Bertoli A, Bilia A, Morelli I . Minor constituents from Bupleurum fruticosum roots. Phytochemistry. 1996; 41(6):1579-82. DOI: 10.1016/0031-9422(95)00749-0. View

16.

Yu J, Tehrim S, Wang L, Dossa K, Zhang X, Ke T . Evolutionary history and functional divergence of the cytochrome P450 gene superfamily between Arabidopsis thaliana and Brassica species uncover effects of whole genome and tandem duplications. BMC Genomics. 2017; 18(1):733. PMC: 5604286. DOI: 10.1186/s12864-017-4094-7. View

17.

Sui C, Zhang J, Wei J, Chen S, Li Y, Xu J . Transcriptome analysis of Bupleurum chinense focusing on genes involved in the biosynthesis of saikosaponins. BMC Genomics. 2011; 12:539. PMC: 3219613. DOI: 10.1186/1471-2164-12-539. View

18.

Ashour M, Wink M . Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action. J Pharm Pharmacol. 2011; 63(3):305-21. PMC: 7197585. DOI: 10.1111/j.2042-7158.2010.01170.x. View

19.

Moses T, Pollier J, Almagro L, Buyst D, Van Montagu M, Pedreno M . Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16α hydroxylase from Bupleurum falcatum. Proc Natl Acad Sci U S A. 2014; 111(4):1634-9. PMC: 3910630. DOI: 10.1073/pnas.1323369111. View

20.

Miettinen K, Pollier J, Buyst D, Arendt P, Csuk R, Sommerwerk S . The ancient CYP716 family is a major contributor to the diversification of eudicot triterpenoid biosynthesis. Nat Commun. 2017; 8:14153. PMC: 5303825. DOI: 10.1038/ncomms14153. View