Different Transcriptional Responses of Haploid and Diploid S. Cerevisiae Strains to Changes in Cofactor Preference of XR

Overview

Journal Microb Cell Fact

Publisher Biomed Central

Specialties Biotechnology
Microbiology

Date 2020 Nov 14

PMID 33187525

Citations 2

Authors

Cai-Yun Xie

Bai-Xue Yang

Qing-Ran Song

Zi-Yuan Xia

Min Gou

Yue-Qin Tang

Affiliations

Soon will be listed here.

Abstract

Background: Xylitol accumulation is a major barrier for efficient ethanol production through heterologous xylose reductase-xylitol dehydrogenase (XR-XDH) pathway in recombinant Saccharomyces cerevisiae. Mutated NADH-preferring XR is usually employed to alleviate xylitol accumulation. However, it remains unclear how mutated XR affects the metabolic network for xylose metabolism. In this study, haploid and diploid strains were employed to investigate the transcriptional responses to changes in cofactor preference of XR through RNA-seq analysis during xylose fermentation.

Results: For the haploid strains, genes involved in xylose-assimilation (XYL1, XYL2, XKS1), glycolysis, and alcohol fermentation had higher transcript levels in response to mutated XR, which was consistent with the improved xylose consumption rate and ethanol yield. For the diploid strains, genes related to protein biosynthesis were upregulated while genes involved in glyoxylate shunt were downregulated in response to mutated XR, which might contribute to the improved yields of biomass and ethanol. When comparing the diploids with the haploids, genes involved in glycolysis and MAPK signaling pathway were significantly downregulated, while oxidative stress related transcription factors (TFs) were significantly upregulated, irrespective of the cofactor preference of XR.

Conclusions: Our results not only revealed the differences in transcriptional responses of the diploid and haploid strains to mutated XR, but also provided underlying basis for better understanding the differences in xylose metabolism between the diploid and haploid strains.

Citing Articles

Oxygenation influences xylose fermentation and gene expression in the yeast genera Spathaspora and Scheffersomyces.

Barros K, Mader M, Krause D, Pangilinan J, Andreopoulos B, Lipzen A Biotechnol Biofuels Bioprod. 2024; 17(1):20.

PMID: 38321504 PMC: 10848558. DOI: 10.1186/s13068-024-02467-8.

Deletion of in a recombinant improved xylose utilization and affected transcription of genes related to amino acid metabolism.

Cheng C, Wang W, Sun M, Tang R, Bai L, Alper H Front Microbiol. 2022; 13:960114.

PMID: 36160216 PMC: 9493327. DOI: 10.3389/fmicb.2022.960114.

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