Put Some Guts into It: Intestinal Organoid Models to Study Viral Infection

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2020 Nov 14

PMID 33187072

Citations 17

Authors

Ines Garcia-Rodriguez

Adithya Sridhar

Dasja Pajkrt

Katja C Wolthers

Affiliations

Soon will be listed here.

Abstract

The knowledge about enteric viral infection has vastly increased over the last eight years due to the development of intestinal organoids and enteroids that suppose a step forward from conventional studies using cell lines. Intestinal organoids and enteroids are three-dimensional (3D) models that closely mimic intestinal cellular heterogeneity and organization. The barrier function within these models has been adapted to facilitate viral studies. In this review, several adaptations (such as organoid-derived two-dimensional (2D) monolayers) and original intestinal 3D models are discussed. The specific advantages and applications, as well as improvements of each model are analyzed and an insight into the possible path for the field is given.

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