Hsa_circ_0006916 Knockdown Represses the Development of Hepatocellular Carcinoma Via Modulating MiR-599/SRSF2 Axis

Overview

Journal Onco Targets Ther

Publisher Dove Medical Press

Specialty Oncology

Date 2020 Nov 12

PMID 33177838

Citations 2

Authors

Zhixiang Zhu

Songhe Shen

Sen Zhao

Zhixue Wang

Affiliations

Soon will be listed here.

Abstract

Background: The aberrantly expressed circular RNAs (circRNAs) are implicated in the progression of hepatocellular carcinoma (HCC). CircRNA hsa_circ_0006916 (circ_0006916) is dysregulated in HCC, but the function and mechanism of this circRNA in HCC development remain uncertain.

Methods: Thirty paired HCC and normal tissues were collected. circ_0006916, microRNA (miR)-599 and serine/arginine rich splicing factor 2 () abundances were examined via quantitative reverse transcription polymerase chain reaction or Western blot. Cell viability, colony ability, migration, invasion, cell cycle and apoptosis were tested via cell counting kit-8, colony formation, wound healing analysis, transwell analysis, and flow cytometry. The interaction between miR-599 and circ_0006916 or was analyzed via dual-luciferase reporter and RNA immunoprecipitation analyses. The function of circ_0006916 on cell growth in vivo was analyzed via xenograft model.

Results: circ_0006916 expression was increased in HCC tissues and cell lines. circ_0006916 knockdown reduced cell viability, colony formation, migration and invasion and caused cell cycle arrest and apoptosis. miR-599 was targeted via circ_0006916, and miR-599 knockdown reversed the influence of circ_0006916 silence on HCC progression. was targeted via miR-599, and miR-599 overexpression suppressed cell viability, colony formation, migration and invasion and promoted cell cycle arrest and apoptosis via decreasing . circ_0006916 could regulate expression via miR-599. circ_0006916 knockdown decreased HCC cell growth in the xenograft model.

Conclusion: circ_0006916 knockdown represses the progression of HCC via regulating miR-599 and .

Citing Articles

Identification of a circRNA-miRNA-mRNA regulatory network for exploring novel therapeutic options for glioma.

He Y, Chen Y, Tong Y, Long W, Liu Q PeerJ. 2021; 9:e11894.

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Current Molecular Biology and Therapeutic Strategy Status and Prospects for circRNAs in HBV-Associated Hepatocellular Carcinoma.

Liao R, Liu L, Zhou J, Wei X, Huang P Front Oncol. 2021; 11:697747.

PMID: 34277444 PMC: 8284075. DOI: 10.3389/fonc.2021.697747.

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