Molecular Characterization and Gene Expression Analysis of the Pro-inflammatory Cytokines IL-1β and IL-8 in the South American Fish Piaractus Mesopotamicus Challenged with Aeromonas Dhakensis

Overview

Journal Genet Mol Biol

Specialty Genetics

Date 2020 Nov 11

PMID 33174977

Citations 5

Authors

Mateus Maldonado Carriero

Flavio Henrique-Silva

Caroline Munhoz Meira

Igor Mateus Queiroz Gato

Alexandre Rodrigues Caetano

Francisco Pereira Lobo

Anderson Luis Alves

Eduardo Sousa Varela

Antonio Augusto Mendes Maia

Affiliations

Soon will be listed here.

Abstract

In the present study, the complete characterization of cDNA and genomic sequences of IL-1β and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1β cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1β, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1β and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups.

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