Genome-wide Survey of Sucrose Non-fermenting 1-related Protein Kinase 2 in Rosaceae and Expression Analysis of PbrSnRK2 in Response to ABA Stress

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2020 Nov 11

PMID 33172386

Citations 7

Authors

Guodong Chen

Jizhong Wang

Xin Qiao

Cong Jin

Weike Duan

Xiaochuan Sun

Juyou Wu

Affiliations

Soon will be listed here.

Abstract

Background: The members of the sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family are specific serine/threonine protein kinases in plants that play important roles in stress signal transduction and adaptation. Because of their positive regulatory roles in response to adverse conditions, the genes encoding thes proteins are considered potential candidates for breeding of plants for disease resistance and genetic improvement. However, there is far less information about this kinase family, and the function of these genes has not been explored in Rosaceae.

Results: A genome-wide survey and analysis of the genes encoding members of the SnRK2 family were performed in pear (Pyrus bretschneideri) and seven other Rosaceae species. A total of 71 SnRK2 genes were identified from the eight Rosaceae species and classified into three subgroups based on phylogenetic analysis and structural characteristics. Purifying selection played a crucial role in the evolution of SnRK2 genes, and whole-genome duplication and dispersed duplication were the primary forces underlying the characteristics of the SnRK2 gene family in Rosaceae. Transcriptome data and qRT-PCR assay results revealed that the distribution of PbrSnRK2s was very extensive, including across the roots, leaves, pollen, styles, and flowers, although most of them were mainly expressed in leaves. In addition, under stress conditions, the transcript levels of some of the genes were upregulated in leaves in response to ABA treatment.

Conclusions: This study provides useful information and a theoretical introduction for the study of the evolution, expression, and functions of the SnRK2 gene family in plants.

Citing Articles

Comprehensive Genomic Analysis of in Rosaceae and Expression Analysis of in Response to Abiotic Stress in .

Wang G, Guan S, Zhu N, Li Q, Chong X, Wang T Plants (Basel). 2023; 12(9).

PMID: 37176842 PMC: 10181103. DOI: 10.3390/plants12091784.

Sucrose non-fermenting1-related protein kinase VcSnRK2.3 promotes anthocyanin biosynthesis in association with VcMYB1 in blueberry.

Wang X, Tang Q, Chi F, Liu H, Zhang H, Song Y Front Plant Sci. 2023; 14:1018874.

PMID: 36909449 PMC: 9998538. DOI: 10.3389/fpls.2023.1018874.

Genome-Wide Identification and Comparative Analysis of the Gene Family in the Rosaceae and Expression Analysis of During Fruit Development.

Zhao B, Yi X, Qiao X, Tang Y, Xu Z, Liu S Front Genet. 2022; 12:792250.

PMID: 35003225 PMC: 8727533. DOI: 10.3389/fgene.2021.792250.

HbSnRK2.6 Functions in ABA-Regulated Cold Stress Response by Promoting HbICE2 Transcriptional Activity in .

Wang X, Liu W, Zeng X, Yan S, Qiu Y, Wang J Int J Mol Sci. 2021; 22(23).

PMID: 34884520 PMC: 8657574. DOI: 10.3390/ijms222312707.

Genome-wide survey of Gγ subunit gene family in eight Rosaceae and expression analysis of PbrGGs in pear (Pyrus bretschneideri).

Chen G, Li Y, Qiao X, Duan W, Jin C, Cheng R BMC Plant Biol. 2021; 21(1):471.

PMID: 34654373 PMC: 8518290. DOI: 10.1186/s12870-021-03250-9.

References

Monks D, Aghoram K, Courtney P, Dewald D, Dewey R . Hyperosmotic stress induces the rapid phosphorylation of a soybean phosphatidylinositol transfer protein homolog through activation of the protein kinases SPK1 and SPK2. Plant Cell. 2001; 13(5):1205-19. PMC: 135558. DOI: 10.1105/tpc.13.5.1205. View

Tang H, Wang X, Bowers J, Ming R, Alam M, Paterson A . Unraveling ancient hexaploidy through multiply-aligned angiosperm gene maps. Genome Res. 2008; 18(12):1944-54. PMC: 2593578. DOI: 10.1101/gr.080978.108. View

Fujita Y, Nakashima K, Yoshida T, Katagiri T, Kidokoro S, Kanamori N . Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. Plant Cell Physiol. 2009; 50(12):2123-32. DOI: 10.1093/pcp/pcp147. View

McLoughlin F, Galvan-Ampudia C, Julkowska M, Caarls L, Van der Does D, Lauriere C . The Snf1-related protein kinases SnRK2.4 and SnRK2.10 are involved in maintenance of root system architecture during salt stress. Plant J. 2012; 72(3):436-49. PMC: 3533798. DOI: 10.1111/j.1365-313X.2012.05089.x. View

Zhou H, Yin H, Chen J, Liu X, Gao Y, Wu J . Gene-expression profile of developing pollen tube of Pyrus bretschneideri. Gene Expr Patterns. 2015; 20(1):11-21. DOI: 10.1016/j.gep.2015.10.004. View

Yoshida R, Umezawa T, Mizoguchi T, Takahashi S, Takahashi F, Shinozaki K . The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis. J Biol Chem. 2005; 281(8):5310-8. DOI: 10.1074/jbc.M509820200. View

Wu J, Wang Z, Shi Z, Zhang S, Ming R, Zhu S . The genome of the pear (Pyrus bretschneideri Rehd.). Genome Res. 2012; 23(2):396-408. PMC: 3561880. DOI: 10.1101/gr.144311.112. View

Merlot S, Vavasseur A, Fenzi F, Giraudat J . Arabidopsis OST1 protein kinase mediates the regulation of stomatal aperture by abscisic acid and acts upstream of reactive oxygen species production. Plant Cell. 2002; 14(12):3089-99. PMC: 151204. DOI: 10.1105/tpc.007906. View

Fujita Y, Yoshida T, Yamaguchi-Shinozaki K . Pivotal role of the AREB/ABF-SnRK2 pathway in ABRE-mediated transcription in response to osmotic stress in plants. Physiol Plant. 2012; 147(1):15-27. DOI: 10.1111/j.1399-3054.2012.01635.x. View

10.

Wang Y, Li X, Lin Y, Wang Y, Wang K, Sun C . Structural Variation, Functional Differentiation, and Activity Correlation of the Cytochrome P450 Gene Superfamily Revealed in Ginseng. Plant Genome. 2018; 11(3). DOI: 10.3835/plantgenome2017.11.0106. View

11.

Ying S, Zhang D, Li H, Liu Y, Shi Y, Song Y . Cloning and characterization of a maize SnRK2 protein kinase gene confers enhanced salt tolerance in transgenic Arabidopsis. Plant Cell Rep. 2011; 30(9):1683-99. DOI: 10.1007/s00299-011-1077-z. View

12.

Maere S, de Bodt S, Raes J, Casneuf T, Van Montagu M, Kuiper M . Modeling gene and genome duplications in eukaryotes. Proc Natl Acad Sci U S A. 2005; 102(15):5454-9. PMC: 556253. DOI: 10.1073/pnas.0501102102. View

13.

Burza A, Pekala I, Sikora J, Siedlecki P, Malagocki P, Bucholc M . Nicotiana tabacum osmotic stress-activated kinase is regulated by phosphorylation on Ser-154 and Ser-158 in the kinase activation loop. J Biol Chem. 2006; 281(45):34299-311. DOI: 10.1074/jbc.M601977200. View

14.

Zheng Z, Xu X, Crosley R, Greenwalt S, Sun Y, Blakeslee B . The protein kinase SnRK2.6 mediates the regulation of sucrose metabolism and plant growth in Arabidopsis. Plant Physiol. 2010; 153(1):99-113. PMC: 2862418. DOI: 10.1104/pp.109.150789. View

15.

Lin Z, Li Y, Zhang Z, Liu X, Hsu C, Du Y . A RAF-SnRK2 kinase cascade mediates early osmotic stress signaling in higher plants. Nat Commun. 2020; 11(1):613. PMC: 6992735. DOI: 10.1038/s41467-020-14477-9. View

16.

Maher C, Stein L, Ware D . Evolution of Arabidopsis microRNA families through duplication events. Genome Res. 2006; 16(4):510-9. PMC: 1457037. DOI: 10.1101/gr.4680506. View

17.

Chen G, Li X, Qiao X, Li J, Wang L, Kou X . Genome-wide survey and expression analysis of the SLAC/SLAH gene family in pear (Pyrus bretschneideri) and other members of the Rosaceae. Genomics. 2019; 111(5):1097-1107. DOI: 10.1016/j.ygeno.2018.07.004. View

18.

Kim M, Park M, Seo P, Song J, Kim H, Park C . Controlled nuclear import of the transcription factor NTL6 reveals a cytoplasmic role of SnRK2.8 in the drought-stress response. Biochem J. 2012; 448(3):353-63. DOI: 10.1042/BJ20120244. View

19.

Chen S, Jia H, Wang X, Shi C, Wang X, Ma P . Hydrogen Sulfide Positively Regulates Abscisic Acid Signaling through Persulfidation of SnRK2.6 in Guard Cells. Mol Plant. 2020; 13(5):732-744. DOI: 10.1016/j.molp.2020.01.004. View

20.

Lee T, Tang H, Wang X, Paterson A . PGDD: a database of gene and genome duplication in plants. Nucleic Acids Res. 2012; 41(Database issue):D1152-8. PMC: 3531184. DOI: 10.1093/nar/gks1104. View