Probiotics Modulate Tilapia Resistance and Immune Response Against Tilapia Lake Virus Infection

Overview

Journal Pathogens

Date 2020 Nov 11

PMID 33172079

Citations 10

Authors

Pitchaporn Waiyamitra

Mehmet Arif Zoral

Aksorn Saengtienchai

Amorn Luengnaruemitchai

Olivier Decamp

Bartolomeo Gorgoglione

Win Surachetpong

Affiliations

Soon will be listed here.

Abstract

Tilapia lake virus (TiLV) causes an emerging viral disease associated with high mortality and economic damage in tilapia farming around the world. The use of probiotics in aquaculture has been suggested as an alternative to antibiotics and drugs to reduce the negative impact of bacterial and viral infections. In this study, we investigate the effect of probiotic spp. supplementation on mortality, viral load, and expression of immune-related genes in red hybrid tilapia ( spp.) upon TiLV infection. Fish were divided into three groups, and fed with: control diet, 0.5% probiotics-supplemented diet, and 1% probiotics-supplemented diet. After 21 days of experimental feeding, the three groups were infected with TiLV and monitored for mortality and growth performances, while organs were sampled at different time points to measure viral load and the transcription modulation of immune response markers. No significant difference was found among the groups in terms of weight gain (WG), average daily gain (ADG), feed efficiency (FE), or feed conversion ratio (FCR). A lower cumulative mortality was retrieved from fish fed 0.5% and 1% probiotics (25% and 24%, respectively), compared to the control group (32%). Moreover, fish fed with 1% probiotic diet had a significantly lower viral load, than those fed with 0.5% probiotic and control diet at 5, 6, 9, and 12 days post infection-challenge (dpc). The expression patterns of immune-related genes, including (also known as CXCL8), , , , (also known as VIPERIN) showed significant upregulation upon probiotic treatment during the peak of TiLV pathogenesis (between 9 and 12 dpc) and during most of the study period in fish fed with 1% probiotics-supplemented diet. Taken together, these findings indicate that dietary supplementation using spp. probiotics may have beneficial effects to strengthen tilapia immunity and resistance against TiLV infections. Therefore, probiotic treatments may be preventively administered to reduce losses caused by this emerging viral infection in tilapia aquaculture.

Citing Articles

Efficacy of Feed Additives on Immune Modulation and Disease Resistance in Tilapia in Coinfection Model with Tilapia Lake Virus and .

Mohamad A, Yamkasem J, Paimeeka S, Khemthong M, Lertwanakarn T, Setthawong P Biology (Basel). 2024; 13(11).

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Metagenomic and metabolomic analysis of changes in intestinal contents of rainbow trout () infected with infectious hematopoietic necrosis virus at different culture water temperatures.

Hai Q, Wang J, Kang W, Cheng S, Li J, Lyu N Front Microbiol. 2023; 14:1275649.

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Potential of Marine Strains of Pseudoalteromonas to Improve Resistance of Juvenile Sea Bass to Pathogens and Limit Biofilm Development.

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PMID: 37851325 DOI: 10.1007/s12602-023-10180-5.

Immune responses to Tilapia lake virus infection: what we know and what we don't know.

Kembou-Ringert J, Steinhagen D, Thompson K, Daly J, Adamek M Front Immunol. 2023; 14:1240094.

PMID: 37622112 PMC: 10445761. DOI: 10.3389/fimmu.2023.1240094.

The Modulation of Immune Responses in -Infected Fish Cells through MAPK/ERK Signalling.

Lertwanakarn T, Khemthong M, Tattiyapong P, Surachetpong W Viruses. 2023; 15(4).

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