Growth Factor-deprived BALB/c 3T3 Murine Fibroblasts Can Enter the S Phase After Induction of C-myc Gene Expression

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1987 Oct 1

PMID 3316981

Citations 10

Authors

F Cavalieri

M Goldfarb

Affiliations

Soon will be listed here.

Abstract

Induction of quiescent BALB/c 3T3 murine fibroblasts by platelet-derived growth factor (PDGF) or fibroblast growth factor (FGFs) is accompanied by induction of c-myc gene expression. To study the role of c-myc in cell growth, we transfected BALB/c 3T3 cells with a plasmid construct containing a glucocorticoid-inducible c-myc gene. When these transfected cells were growth arrested in PDGF-FGF-freedefined medium, glucocorticoid treatment induced S-phase DNA synthesis. This induction of DNA synthesis was inefficient, and cell proliferation was not evident, suggesting that growth factors act through stimulation of c-myc expression together with other intracellular events.

Citing Articles

Skp2 is necessary for Myc-induced keratinocyte proliferation but dispensable for Myc oncogenic activity in the oral epithelium.

Sistrunk C, Macias E, Nakayama K, Kim Y, Rodriguez-Puebla M Am J Pathol. 2011; 178(6):2470-7.

PMID: 21641375 PMC: 3124025. DOI: 10.1016/j.ajpath.2011.02.034.

β-Catenin-dependent FGF signaling sustains cell survival in the anterior embryonic head by countering Smad4.

Paek H, Hwang J, Zukin R, Hebert J Dev Cell. 2011; 20(5):689-99.

PMID: 21571225 PMC: 3099549. DOI: 10.1016/j.devcel.2011.04.010.

Skp2 directs Myc-mediated suppression of p27Kip1 yet has modest effects on Myc-driven lymphomagenesis.

Old J, Kratzat S, Hoellein A, Graf S, Nilsson J, Nilsson L Mol Cancer Res. 2010; 8(3):353-62.

PMID: 20197382 PMC: 3095030. DOI: 10.1158/1541-7786.MCR-09-0232.

c-Myc augments gamma irradiation-induced apoptosis by suppressing Bcl-XL.

Maclean K, Keller U, Rodriguez-Galindo C, Nilsson J, Cleveland J Mol Cell Biol. 2003; 23(20):7256-70.

PMID: 14517295 PMC: 230315. DOI: 10.1128/MCB.23.20.7256-7270.2003.

The tmp gene, encoding a membrane protein, is a c-Myc target with a tumorigenic activity.

Ben-Porath I, Yanuka O, Benvenisty N Mol Cell Biol. 1999; 19(5):3529-39.

PMID: 10207076 PMC: 84145. DOI: 10.1128/MCB.19.5.3529.

References

Erikson J, Drwinga H, Nowell P, Croce C . Transcriptional activation of the translocated c-myc oncogene in burkitt lymphoma. Proc Natl Acad Sci U S A. 1983; 80(3):820-4. PMC: 393472. DOI: 10.1073/pnas.80.3.820. View

Nishikura K, Murray J . Antisense RNA of proto-oncogene c-fos blocks renewed growth of quiescent 3T3 cells. Mol Cell Biol. 1987; 7(2):639-49. PMC: 365119. DOI: 10.1128/mcb.7.2.639-649.1987. View

Stanton L, Watt R, Marcu K . Translocation, breakage and truncated transcripts of c-myc oncogene in murine plasmacytomas. Nature. 1983; 303(5916):401-6. DOI: 10.1038/303401a0. View

Kelly K, Cochran B, Stiles C, Leder P . Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell. 1983; 35(3 Pt 2):603-10. DOI: 10.1016/0092-8674(83)90092-2. View

Slamon D, DeKernion J, Verma I, Cline M . Expression of cellular oncogenes in human malignancies. Science. 1984; 224(4646):256-62. DOI: 10.1126/science.6538699. View

Armelin H, Armelin M, Kelly K, Stewart T, Leder P, Cochran B . Functional role for c-myc in mitogenic response to platelet-derived growth factor. Nature. 1984; 310(5979):655-60. DOI: 10.1038/310655a0. View

Greenberg M, Ziff E . Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature. 1984; 311(5985):433-8. DOI: 10.1038/311433a0. View

Melton D, Krieg P, Rebagliati M, Maniatis T, Zinn K, Green M . Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984; 12(18):7035-56. PMC: 320141. DOI: 10.1093/nar/12.18.7035. View

Sharp P, Gallimore P, Flint S . Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harb Symp Quant Biol. 1975; 39 Pt 1:457-74. DOI: 10.1101/sqb.1974.039.01.058. View

10.

Wigler M, Sweet R, Sim G, Wold B, Pellicer A, Lacy E . Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell. 1979; 16(4):777-85. DOI: 10.1016/0092-8674(79)90093-x. View

11.

Wang T, Korn D . Reactivity of KB cell deoxyribonucleic acid polymerases alpha and beta with nicked and gapped deoxyribonucleic acid. Biochemistry. 1980; 19(9):1782-90. DOI: 10.1021/bi00550a009. View

12.

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

13.

Huberman J . New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha. Cell. 1981; 23(3):647-8. DOI: 10.1016/0092-8674(81)90426-8. View

14.

Lee F, Mulligan R, Berg P, Ringold G . Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids. Nature. 1981; 294(5838):228-32. DOI: 10.1038/294228a0. View

15.

Abrams H, Rohrschneider L, Eisenman R . Nuclear location of the putative transforming protein of avian myelocytomatosis virus. Cell. 1982; 29(2):427-39. DOI: 10.1016/0092-8674(82)90159-3. View

16.

Colby W, Chen E, Smith D, Levinson A . Identification and nucleotide sequence of a human locus homologous to the v-myc oncogene of avian myelocytomatosis virus MC29. Nature. 1983; 301(5902):722-5. DOI: 10.1038/301722a0. View

17.

Goyette M, Petropoulos C, Shank P, Fausto N . Regulated transcription of c-Ki-ras and c-myc during compensatory growth of rat liver. Mol Cell Biol. 1984; 4(8):1493-8. PMC: 368939. DOI: 10.1128/mcb.4.8.1493-1498.1984. View

18.

Fasano O, Birnbaum D, Edlund L, Fogh J, Wigler M . New human transforming genes detected by a tumorigenicity assay. Mol Cell Biol. 1984; 4(9):1695-705. PMC: 368975. DOI: 10.1128/mcb.4.9.1695-1705.1984. View

19.

Thompson C, Challoner P, Neiman P, Groudine M . Levels of c-myc oncogene mRNA are invariant throughout the cell cycle. Nature. 1985; 314(6009):363-6. DOI: 10.1038/314363a0. View

20.

Hann S, Thompson C, Eisenman R . c-myc oncogene protein synthesis is independent of the cell cycle in human and avian cells. Nature. 1985; 314(6009):366-9. DOI: 10.1038/314366a0. View