Evaluating the Value of Day 0 of an ICSI Cycle on Indicating Laboratory Outcome

Overview

Journal Sci Rep

Specialty Science

Date 2020 Nov 10

PMID 33168856

Citations 1

Authors

E Maziotis

K Sfakianoudis

P Giannelou

S Grigoriadis

A Rapani

P Tsioulou

K Nikolettos

A Pantou

A Tiptiri-Kourpeti

M Koutsilieris

B Asimakopoulos

N Nikolettos

K Pantos

Mara Simopoulou

Affiliations

Soon will be listed here.

Abstract

A number of oocyte characteristics have been associated with fertilization, implantation and live-birth rates, albeit without reaching a consensus. This study aims to delineate possible associations between oocyte characteristics, oocyte behavior during intracytoplasmic sperm injection (ICSI), fertilization potential, and laboratory outcomes. Four-hundred and seventy-seven patients, yielding 3452 oocytes, were enrolled in this prospective observational study from 2015 to 2018. Οoplasm granularity was associated with poor embryo quality and higher probabilities of post-ICSI oocytes and embryos discarded in any developmental stage and never selected for embryo transfer or cryopreservation (p < 0.001). Both sudden or difficult ooplasm aspiration, and high or lack of resistance during ICSI were associated with either a poor Zygote-Score or fertilization failure (p < 0.001). Sudden or difficult ooplasm aspiration and high resistance during ICSI penetration were positively associated with resulting to a post-ICSI oocyte or embryo that would be selected for discard. Evaluation of oocyte characteristics and oocyte behavior during ICSI may provide early information regarding laboratory and cycle outcomes. Particularly, ooplasm granularity, and fragmentation of polar body, along with sudden or difficult ooplasm aspiration and high or lack of resistance during ICSI penetration may hinder the outcome of an ICSI cycle. The associations presented herein may contribute towards development of a grading system or a prediction model. Taking into account information on oocytes and ICSI behavior may effectively assist in enhancing IVF outcome rates.

Citing Articles

Molecular Drivers of Developmental Arrest in the Human Preimplantation Embryo: A Systematic Review and Critical Analysis Leading to Mapping Future Research.

Sfakianoudis K, Maziotis E, Karantzali E, Kokkini G, Grigoriadis S, Pantou A Int J Mol Sci. 2021; 22(15).

PMID: 34361119 PMC: 8347543. DOI: 10.3390/ijms22158353.

References

Petersen B, Boel M, Montag M, Gardner D . Development of a generally applicable morphokinetic algorithm capable of predicting the implantation potential of embryos transferred on Day 3. Hum Reprod. 2016; 31(10):2231-44. PMC: 5027927. DOI: 10.1093/humrep/dew188. View

Yu E, Ahn H, Lee J, Jee B, Kim S . Fertilization and embryo quality of mature oocytes with specific morphological abnormalities. Clin Exp Reprod Med. 2016; 42(4):156-62. PMC: 4724600. DOI: 10.5653/cerm.2015.42.4.156. View

Pantou A, Simopoulou M, Sfakianoudis K, Giannelou P, Rapani A, Maziotis E . The Role of Laparoscopic Investigation in Enabling Natural Conception and Avoiding in vitro Fertilization Overuse for Infertile Patients of Unidentified Aetiology and Recurrent Implantation Failure Following in vitro Fertilization. J Clin Med. 2019; 8(4). PMC: 6517944. DOI: 10.3390/jcm8040548. View

Simopoulou M, Sfakianoudis K, Antoniou N, Maziotis E, Rapani A, Bakas P . Making IVF more effective through the evolution of prediction models: is prognosis the missing piece of the puzzle?. Syst Biol Reprod Med. 2018; 64(5):305-323. DOI: 10.1080/19396368.2018.1504347. View

Ebner T, Maurer M, Shebl O, Moser M, Mayer R, Duba H . Planar embryos have poor prognosis in terms of blastocyst formation and implantation. Reprod Biomed Online. 2012; 25(3):267-72. DOI: 10.1016/j.rbmo.2012.05.007. View

Farhi J, Cohen K, Mizrachi Y, Weissman A, Raziel A, Orvieto R . Should ICSI be implemented during IVF to all advanced-age patients with non-male factor subfertility?. Reprod Biol Endocrinol. 2019; 17(1):30. PMC: 6407274. DOI: 10.1186/s12958-019-0474-y. View

Swain J, Pool T . ART failure: oocyte contributions to unsuccessful fertilization. Hum Reprod Update. 2008; 14(5):431-46. DOI: 10.1093/humupd/dmn025. View

Armstrong S, Bhide P, Jordan V, Pacey A, Marjoribanks J, Farquhar C . Time-lapse systems for embryo incubation and assessment in assisted reproduction. Cochrane Database Syst Rev. 2019; 5:CD011320. PMC: 6539473. DOI: 10.1002/14651858.CD011320.pub4. View

Rosen M, Shen S, Dobson A, Fujimoto V, McCulloch C, Cedars M . Oocyte degeneration after intracytoplasmic sperm injection: a multivariate analysis to assess its importance as a laboratory or clinical marker. Fertil Steril. 2006; 85(6):1736-43. DOI: 10.1016/j.fertnstert.2005.12.017. View

10.

Vanderzwalmen P, Bertin G, Lejeune B, Nijs M, Vandamme B, Schoysman R . Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema. Hum Reprod. 1996; 11(3):540-7. DOI: 10.1093/humrep/11.3.540. View

11.

Rose B, Laky D . Polar body fragmentation in IVM oocytes is associated with impaired fertilization and embryo development. J Assist Reprod Genet. 2013; 30(5):679-82. PMC: 3663962. DOI: 10.1007/s10815-013-9982-4. View

12.

Balaban B, Urman B . Effect of oocyte morphology on embryo development and implantation. Reprod Biomed Online. 2006; 12(5):608-15. DOI: 10.1016/s1472-6483(10)61187-x. View

13.

Ebner T, Yaman C, Moser M, Sommergruber M, Feichtinger O, Tews G . Prognostic value of first polar body morphology on fertilization rate and embryo quality in intracytoplasmic sperm injection. Hum Reprod. 2000; 15(2):427-30. DOI: 10.1093/humrep/15.2.427. View

14.

Shi W, Xu B, Wu L, Jin R, Luan H, Luo L . Oocytes with a dark zona pellucida demonstrate lower fertilization, implantation and clinical pregnancy rates in IVF/ICSI cycles. PLoS One. 2014; 9(2):e89409. PMC: 3933533. DOI: 10.1371/journal.pone.0089409. View

15.

Wu Y, Lazzaroni-Tealdi E, Wang Q, Zhang L, Barad D, Kushnir V . Different effectiveness of closed embryo culture system with time-lapse imaging (EmbryoScope(TM)) in comparison to standard manual embryology in good and poor prognosis patients: a prospectively randomized pilot study. Reprod Biol Endocrinol. 2016; 14(1):49. PMC: 4995783. DOI: 10.1186/s12958-016-0181-x. View

16.

Yanez L, Han J, Behr B, Pera R, Camarillo D . Human oocyte developmental potential is predicted by mechanical properties within hours after fertilization. Nat Commun. 2016; 7:10809. PMC: 4770082. DOI: 10.1038/ncomms10809. View

17.

Rubino P, Vigano P, Luddi A, Piomboni P . The ICSI procedure from past to future: a systematic review of the more controversial aspects. Hum Reprod Update. 2015; 22(2):194-227. DOI: 10.1093/humupd/dmv050. View

18.

Bingol B, Abike F, Gedikbasi A, Tapisiz O, Gunenc Z . Comparison of chromosomal abnormality rates in ICSI for non-male factor and spontaneous conception. J Assist Reprod Genet. 2011; 29(1):25-30. PMC: 3252418. DOI: 10.1007/s10815-011-9646-1. View

19.

De Vos A, Van de Velde H, Joris H, Verheyen G, Devroey P, Van Steirteghem A . Influence of individual sperm morphology on fertilization, embryo morphology, and pregnancy outcome of intracytoplasmic sperm injection. Fertil Steril. 2003; 79(1):42-8. DOI: 10.1016/s0015-0282(02)04571-5. View

20.

Catford S, McLachlan R, OBryan M, Halliday J . Long-term follow-up of ICSI-conceived offspring compared with spontaneously conceived offspring: a systematic review of health outcomes beyond the neonatal period. Andrology. 2018; 6(5):635-653. DOI: 10.1111/andr.12526. View