3-O-sulfated Heparan Sulfate Interactors Target Synaptic Adhesion Molecules from Neonatal Mouse Brain and Inhibit Neural Activity and Synaptogenesis in Vitro

Overview

Journal Sci Rep

Specialty Science

Date 2020 Nov 6

PMID 33154448

Citations 7

Authors

Auriane Maiza

Nazha Sidahmed-Adrar

Patrick P Michel

Gilles Carpentier

Damien Habert

Carine Dalle

Walid Redouane

Magda Hamza

T H van Kuppevelt

Mohand Ouidir Ouidja

Jose Courty

Sandrine Chantepie

Dulce Papy-Garcia

Olivier Stettler

Affiliations

Soon will be listed here.

Abstract

Heparan sulfate (HS) chains, covalently linked to heparan sulfate proteoglycans (HSPG), promote synaptic development and functions by connecting various synaptic adhesion proteins (AP). HS binding to AP could vary according to modifications of HS chains by different sulfotransferases. 3-O-sulfotransferases (Hs3sts) produce rare 3-O-sulfated HSs (3S-HSs), of poorly known functions in the nervous system. Here, we showed that a peptide known to block herpes simplex virus by interfering with 3S-HSs in vitro and in vivo (i.e. G2 peptide), specifically inhibited neural activity, reduced evoked glutamate release, and impaired synaptic assembly in hippocampal cell cultures. A role for 3S-HSs in promoting synaptic assembly and neural activity is consistent with the synaptic interactome of G2 peptide, and with the detection of Hs3sts and their products in synapses of cultured neurons and in synaptosomes prepared from developing brains. Our study suggests that 3S-HSs acting as receptors for herpesviruses might be important regulators of neuronal and synaptic development in vertebrates.

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