A Single-cell Analysis of the Molecular Lineage of Chordate Embryogenesis

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2020 Nov 5

PMID 33148647

Citations 9

Authors

Tengjiao Zhang

Yichi Xu

Kaoru Imai

Teng Fei

Guilin Wang

Bo Dong

Tianwei Yu

Yutaka Satou

Weiyang Shi

Zhirong Bao

Affiliations

Soon will be listed here.

Abstract

Progressive unfolding of gene expression cascades underlies diverse embryonic lineage development. Here, we report a single-cell RNA sequencing analysis of the complete and invariant embryonic cell lineage of the tunicate from fertilization to the onset of gastrulation. We reconstructed a developmental landscape of 47 cell types over eight cell cycles in the wild-type embryo and identified eight fate transformations upon fibroblast growth factor (FGF) inhibition. For most FGF-dependent asymmetric cell divisions, the bipotent mother cell displays the gene signature of the default daughter fate. In convergent differentiation of the two notochord lineages, we identified additional gene pathways parallel to the master regulator / Last, we showed that the defined cell types can be matched to E6.5-E8.5 stage mouse cell types and display conserved expression of limited number of transcription factors. This study provides a high-resolution single-cell dataset to understand chordate early embryogenesis and cell lineage differentiation.

Citing Articles

Hemichordate cis-regulatory genomics and the gene expression dynamics of deuterostomes.

Perez-Posada A, Lin C, Fan T, Lin C, Chen Y, Gomez-Skarmeta J Nat Ecol Evol. 2024; 8(12):2213-2227.

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Quantitative proteome dynamics across embryogenesis in a model chordate.

Frese A, Mariossi A, Levine M, Wuhr M iScience. 2024; 27(4):109355.

PMID: 38510129 PMC: 10951915. DOI: 10.1016/j.isci.2024.109355.

Temporospatial hierarchy and allele-specific expression of zygotic genome activation revealed by distant interspecific urochordate hybrids.

Wei J, Zhang W, Jiang A, Peng H, Zhang Q, Li Y Nat Commun. 2024; 15(1):2395.

PMID: 38493164 PMC: 10944513. DOI: 10.1038/s41467-024-46780-0.

The specious art of single-cell genomics.

Chari T, Pachter L PLoS Comput Biol. 2023; 19(8):e1011288.

PMID: 37590228 PMC: 10434946. DOI: 10.1371/journal.pcbi.1011288.

Marine Invertebrates One Cell at A Time: Insights from Single-Cell Analysis.

Bump P, Lubeck L Integr Comp Biol. 2023; 63(5):999-1009.

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