7-HYB, a Phenolic Compound Isolated from Houtt Increases Cell Migration, Osteoblast Differentiation, and Mineralization Through BMP2 and β-catenin Signaling

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Nov 3

PMID 33137925

Citations 7

Authors

Kyung-Ran Park

Yoon-Ju Kwon

Ji-Eun Park

Hyung-Mun Yun

Affiliations

Soon will be listed here.

Abstract

The seeds (nutmegs) of Houtt have been used as popular spices and traditional medicine to treat a variety of diseases. A phenolic compound, ((7S)-8'-(benzo[3',4']dioxol-1'-yl)-7-hydroxypropyl)benzene-2,4-diol (7-HYB) was isolated from the seeds of . This study aimed to investigate the anabolic effects of 7-HYB in osteogenesis and bone mineralization. In the present study, 7-HYB promotes the early and late differentiation of MC3T3-E1 preosteoblasts. 7-HYB also elevated cell migration rate during differentiation of the preosteoblasts with the increased phosphorylation of mitogen-activated protein kinases (MAPKs) including ERK1/2, p38, and JNK. In addition, 7-HYB induced the protein level of BMP2, the phosphorylation of Smad1/5/8, and the expression of RUNX2. 7-HYB also inhibited GSK3β and subsequently increased the level of β-catenin. However, in bone marrow macrophages (BMMs), 7-HYB has no biological effects in cell viability, TRAP-positive multinuclear osteoclasts, and gene expression (c-Fos and NF-ATc1) in receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis. Our findings suggest that 7-HYB plays an important role in osteoblast differentiation through the BMP2 and β-catenin signaling pathway. It also indicates that 7-HYB might have a therapeutic effect for the treatment of bone diseases such as osteoporosis and periodontitis.

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References

Park K, Lee H, Cho M, Yun H . A Phytochemical Constituent, (E)-Methyl-Cinnamate Isolated from Hayata Suppresses Cell Survival, Migration, and Differentiation in Pre-Osteoblasts. Int J Mol Sci. 2020; 21(10). PMC: 7279157. DOI: 10.3390/ijms21103700. View

Gaur T, Lengner C, Hovhannisyan H, Bhat R, Bodine P, Komm B . Canonical WNT signaling promotes osteogenesis by directly stimulating Runx2 gene expression. J Biol Chem. 2005; 280(39):33132-40. DOI: 10.1074/jbc.M500608200. View

Park K, Yun H . RANKL-induced osteoclastogenesis in bone marrow-derived macrophages is suppressed by cisapride. Toxicology. 2019; 422:95-101. DOI: 10.1016/j.tox.2019.05.010. View

Van Gils C, Cox P . Ethnobotany of nutmeg in the Spice Islands. J Ethnopharmacol. 1994; 42(2):117-24. DOI: 10.1016/0378-8741(94)90105-8. View

Park K, Kim E, Hong J, Yun H . Dysregulation of 5-hydroxytryptamine 6 receptor accelerates maturation of bone-resorbing osteoclasts and induces bone loss. Theranostics. 2018; 8(11):3087-3098. PMC: 5996355. DOI: 10.7150/thno.24426. View

Day T, Guo X, Garrett-Beal L, Yang Y . Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis. Dev Cell. 2005; 8(5):739-50. DOI: 10.1016/j.devcel.2005.03.016. View

Khosla S, Riggs B . Pathophysiology of age-related bone loss and osteoporosis. Endocrinol Metab Clin North Am. 2005; 34(4):1015-30, xi. DOI: 10.1016/j.ecl.2005.07.009. View

Canalis E, Economides A, Gazzerro E . Bone morphogenetic proteins, their antagonists, and the skeleton. Endocr Rev. 2003; 24(2):218-35. DOI: 10.1210/er.2002-0023. View

Cuong T, Manh Hung T, Na M, Ha D, Kim J, Lee D . Inhibitory effect on NO production of phenolic compounds from Myristica fragrans. Bioorg Med Chem Lett. 2011; 21(22):6884-7. DOI: 10.1016/j.bmcl.2011.09.021. View

10.

Marie P . Osteoblast dysfunctions in bone diseases: from cellular and molecular mechanisms to therapeutic strategies. Cell Mol Life Sci. 2014; 72(7):1347-61. PMC: 11113967. DOI: 10.1007/s00018-014-1801-2. View

11.

Kim M, Song Y, Hwang J . Kirenol stimulates osteoblast differentiation through activation of the BMP and Wnt/β-catenin signaling pathways in MC3T3-E1 cells. Fitoterapia. 2014; 98:59-65. DOI: 10.1016/j.fitote.2014.07.013. View

12.

Lee H, Jung E, Bae S, Kwon K, Kim J, Suh H . Stimulation of osteoblastic differentiation and mineralization in MC3T3-E1 cells by yeast hydrolysate. Phytother Res. 2010; 25(5):716-23. DOI: 10.1002/ptr.3328. View

13.

Katagiri T, Yamaguchi A, Komaki M, Abe E, Takahashi N, Ikeda T . Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage. J Cell Biol. 1994; 127(6 Pt 1):1755-66. PMC: 2120318. DOI: 10.1083/jcb.127.6.1755. View

14.

Wozney J, Rosen V, Celeste A, Mitsock L, Whitters M, Kriz R . Novel regulators of bone formation: molecular clones and activities. Science. 1988; 242(4885):1528-34. DOI: 10.1126/science.3201241. View

15.

Ghiasi M, Chen J, Vaziri A, Rodriguez E, Nazarian A . Bone fracture healing in mechanobiological modeling: A review of principles and methods. Bone Rep. 2017; 6:87-100. PMC: 5365304. DOI: 10.1016/j.bonr.2017.03.002. View

16.

Barceloux D . Nutmeg (Myristica fragrans Houtt.). Dis Mon. 2009; 55(6):373-9. DOI: 10.1016/j.disamonth.2009.03.007. View

17.

Marie P, Kassem M . Osteoblasts in osteoporosis: past, emerging, and future anabolic targets. Eur J Endocrinol. 2011; 165(1):1-10. DOI: 10.1530/EJE-11-0132. View

18.

Chang Y, Shih Y, Chen Y, Liu C, Fang W, Tsai C . Schwann Cell Migration Induced by Earthworm Extract via Activation of PAs and MMP2/9 Mediated through ERK1/2 and p38. Evid Based Complement Alternat Med. 2009; 2011:395458. PMC: 3135425. DOI: 10.1093/ecam/nep131. View

19.

Fukuda T, Kokabu S, Ohte S, Sasanuma H, Kanomata K, Yoneyama K . Canonical Wnts and BMPs cooperatively induce osteoblastic differentiation through a GSK3beta-dependent and beta-catenin-independent mechanism. Differentiation. 2010; 80(1):46-52. DOI: 10.1016/j.diff.2010.05.002. View

20.

Vondracek S, Minne P, McDermott M . Clinical challenges in the management of osteoporosis. Clin Interv Aging. 2008; 3(2):315-29. PMC: 2546475. DOI: 10.2147/cia.s2539. View