Molecular Study of Human Astrovirus in Egyptian Children with Acute Gastroenteritis

Overview

Journal Germs

Specialty Infectious Diseases

Date 2020 Nov 2

PMID 33134194

Citations 2

Authors

Maysaa El Sayed Zaki

Ghada El-Saeed Mashaly

Mona Abdel Latif AlSayed

Manal Mahmoud Nomir

Affiliations

Soon will be listed here.

Abstract

Introduction: Human astrovirus (HAstV) has been increasingly identified as an important cause of acute gastroenteritis in young children. Limited information is available about the prevalence and genotype distribution of classic HAstV causing acute gastroenteritis in Egyptian children.

Methods: Stool samples were collected from 100 infants and children attending the gastroenterology outpatient clinic in Mansoura University Children Hospital and suffering from acute gastroenteritis during the period extending from January 2018 to January 2019. Samples were tested for HAstV using reverse transcription PCR. Genotyping was performed using type-specific reverse transcription nested PCR.

Results: Among 100 children included in this study, the detection rate of HAstV was 11% (11 patients). There was a significant difference regarding age between cases positive and negative for HAstV (p=0.005). There was a higher prevalence of HAstV in children aged one year or younger. Significant association was detected between HAstV positive cases and rural residence (p=0.002), summer season (p=0.025) and fever (p=0.017). The HAstV genotypes detected were HAstV-8 (8/11, 72.7%), HAstV-3 (2/11, 18.2%) and HAstV-2 (1/11, 9.1%).

Conclusions: This study suggests that HAstV is a common pathogen causing gastroenteritis in Egyptian children especially in rural areas. The most frequent HAstV genotype in our study was HAstV-8.

Citing Articles

High Burden of Co-Infection with Multiple Enteric Pathogens in Children Suffering with Diarrhoea from Rural and Peri-Urban Communities in South Africa.

Potgieter N, Heine L, Ngandu J, Ledwaba S, Zitha T, Mudau L Pathogens. 2023; 12(2).

PMID: 36839587 PMC: 9959912. DOI: 10.3390/pathogens12020315.

Viral pathogens of acute gastroenteritis in Egyptian children: role of the parechovirus.

Mashaly M, Alkasaby N, Bakr A, Zaki M, Montasser K BMC Infect Dis. 2022; 22(1):584.

PMID: 35768762 PMC: 9245302. DOI: 10.1186/s12879-022-07562-5.

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