Sp. Nov. E24 Isolated From the Oropharynx of Healthy Chinese Children

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2020 Nov 2

PMID 33133039

Citations 3

Authors

Yang Zou

Ye Sun

He Qi

Defeng Liu

Han Tian

Nan Wang

Xinming Li

Affiliations

Soon will be listed here.

Abstract

A Gram-positive, α-hemolytic, catalase-negative, facultative anaerobic and non-motile coccus was isolated form throat swabs taken from the oropharynx of healthy children. The genome was shown to be 1.950,659 bp long and contained 42.03 mol% G + C bases with 1,942 protein-coding and 53 RNA coding genes. The results of 16S rRNA gene sequencing strongly suggested that the strain is a member of the genus, with 98.04, 98.11, and 97.34% similarities to ATCC 700641, LMG 27207 and ATCC 15912, respectively. A sodA gene comparison exhibited a sequence identity of 92.6% with the closest strain ATCC 700641. DNA-DNA hybridization showed a highest DNA similarity value of 52% with ATCC 700641. Comparing 18 biochemical traits, the similarities of the strain E24 were 72% with LMG 27207, 78% with ATCC 700641 and 44% with ATCC 15912. We suggest that based on the genotypic and phenotypic results that the strain E24 is a novel species of the genus and propose the name sp. nov. E24.

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