SELEX-based DNA Aptamer Selection: A Perspective from the Advancement of Separation Techniques

Overview

Journal Anal Sci

Publisher Springer

Specialty Chemistry

Date 2020 Nov 2

PMID 33132238

Citations 24

Authors

Shingo Saito

Affiliations

Soon will be listed here.

Abstract

DNA aptamers, which are short, single-stranded DNA sequences that selectively bind to target substances (proteins, cells, small molecules, metal ions), can be acquired by means of the systematic evolution of ligands by exponential enrichment (SELEX) methodology. In the SELEX procedure, one of the keys for the effective acquisition of high-affinity and functional aptamer sequences is the separation stage to isolate target-bound DNA from unbound DNA in a randomized DNA library. In this review, various remarkable advancements in separation techniques for SELEX-based aptamer selection developed in this decade, are described and discussed, including CE-, microfluidic chip-, solid phase-, and FACS-based SELEX along with other methods.

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