SINEUP Long Non-coding RNA Acts Via PTBP1 and HNRNPK to Promote Translational Initiation Assemblies

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2020 Nov 1

PMID 33130894

Citations 29

Authors

Naoko Toki

Hazuki Takahashi

Harshita Sharma

Matthew N Z Valentine

Ferdous-Ur M Rahman

Silvia Zucchelli

Stefano Gustincich

Piero Carninci

Affiliations

Soon will be listed here.

Abstract

SINEUPs are long non-coding RNAs (lncRNAs) that contain a SINE element, and which up-regulate the translation of target mRNA. They have been studied in a wide range of applications, as both biological and therapeutic tools, although the underpinning molecular mechanism is unclear. Here, we focused on the sub-cellular distribution of target mRNAs and SINEUP RNAs, performing co-transfection of expression vectors for these transcripts into human embryonic kidney cells (HEK293T/17), to investigate the network of translational regulation. The results showed that co-localization of target mRNAs and SINEUP RNAs in the cytoplasm was a key phenomenon. We identified PTBP1 and HNRNPK as essential RNA binding proteins. These proteins contributed to SINEUP RNA sub-cellular distribution and to assembly of translational initiation complexes, leading to enhanced target mRNA translation. These findings will promote a better understanding of the mechanisms employed by regulatory RNAs implicated in efficient protein translation.

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