Effects of Human Endothelial Progenitor Cell and Its Conditioned Medium on Oocyte Development and Subsequent Embryo Development

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Oct 30

PMID 33121114

Citations 7

Authors

Seok Hee Lee

Affiliations

Soon will be listed here.

Abstract

Human endothelial progenitor cells (EPCs) secrete numerous growth factors, and they have been applied to regenerative medicine for their roles in angiogenesis as well as neovascularization. Angiogenesis is one of the essential factors for the maturation of ovarian follicles; however, the physiological function of EPCs or their derivatives on in vitro culture systems has not been fully understood. The aim of this study was to evaluate the effectiveness of EPCs and their conditioned medium (EPC-CM) on oocyte development and subsequent embryo development. In the results, the oocyte development and subsequent embryo development were significantly improved in EPCs and the EPC-CM group. In addition, markedly increased levels of growth factors/cytokines, such as basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), insulin growth factor-1 (IGF-1), interleukin-10 (IL-10), and epidermal growth factor (EGF), were observed in medium from the EPC-CM group. Additionally, EPC-CM after in vitro maturation (IVM) had significantly decreased reactive oxygen species (ROS) levels compared to those of other groups. Transcriptional levels of growth factor receptor-related genes (, ) and anti-apoptotic-related gene () were significantly upregulated in cumulus cells/oocytes from the EPC-CM group compared with those from the control. Furthermore, the expression levels of cumulus expansion-related genes (, , ) and oocyte-maturation-related factors (, ) were significantly enhanced in the EPC-CM group. Consequently, the present study provides the first evidence that EPC-CM contains several essential growth factors for oocyte development by regulating genes involved in oocyte maturation.

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References

Kollmann Z, Schneider S, Fux M, Bersinger N, von Wolff M . Gonadotrophin stimulation in IVF alters the immune cell profile in follicular fluid and the cytokine concentrations in follicular fluid and serum. Hum Reprod. 2017; 32(4):820-831. DOI: 10.1093/humrep/dex005. View

Zhang M, Malik A, Rehman J . Endothelial progenitor cells and vascular repair. Curr Opin Hematol. 2014; 21(3):224-8. PMC: 4090051. DOI: 10.1097/MOH.0000000000000041. View

Ornitz D, Itoh N . Fibroblast growth factors. Genome Biol. 2001; 2(3):REVIEWS3005. PMC: 138918. DOI: 10.1186/gb-2001-2-3-reviews3005. View

Du S, Liu X, Deng K, Zhou W, Lu F, Shi D . The expression pattern of fibroblast growth factor 10 and its receptors during buffalo follicular development. Int J Clin Exp Pathol. 2020; 11(10):4934-4941. PMC: 6962906. View

Sagirkaya H, Misirlioglu M, Kaya A, First N, Parrish J, Memili E . Developmental potential of bovine oocytes cultured in different maturation and culture conditions. Anim Reprod Sci. 2006; 101(3-4):225-40. DOI: 10.1016/j.anireprosci.2006.09.016. View

Bendall S, Hughes C, Campbell J, Stewart M, Pittock P, Liu S . An enhanced mass spectrometry approach reveals human embryonic stem cell growth factors in culture. Mol Cell Proteomics. 2008; 8(3):421-32. PMC: 2649806. DOI: 10.1074/mcp.M800190-MCP200. View

Silveira J, Winger Q, Bouma G, Carnevale E . Effects of age on follicular fluid exosomal microRNAs and granulosa cell transforming growth factor-β signalling during follicle development in the mare. Reprod Fertil Dev. 2015; 27(6):897-905. DOI: 10.1071/RD14452. View

Lee S, Oh H, Kim M, Kim G, Choi Y, Jo Y . Oocyte maturation-related gene expression in the canine oviduct, cumulus cells, and oocytes and effect of co-culture with oviduct cells on in vitro maturation of oocytes. J Assist Reprod Genet. 2017; 34(7):929-938. PMC: 5476537. DOI: 10.1007/s10815-017-0910-x. View

Su Y, Wu X, OBrien M, Pendola F, Denegre J, Matzuk M . Synergistic roles of BMP15 and GDF9 in the development and function of the oocyte-cumulus cell complex in mice: genetic evidence for an oocyte-granulosa cell regulatory loop. Dev Biol. 2004; 276(1):64-73. DOI: 10.1016/j.ydbio.2004.08.020. View

10.

Giudice L . Insulin-like growth factors and ovarian follicular development. Endocr Rev. 1992; 13(4):641-69. DOI: 10.1210/edrv-13-4-641. View

11.

Morillo V, Akthar I, Fiorenza M, Takahashi K, Sasaki M, Marey M . Toll-like receptor 2 mediates the immune response of the bovine oviductal ampulla to sperm binding. Mol Reprod Dev. 2020; 87(10):1059-1069. DOI: 10.1002/mrd.23422. View

12.

Lee S, Ra J, Oh H, Kim M, Setyawan E, Choi Y . Clinical Assessment of Intravenous Endothelial Progenitor Cell Transplantation in Dogs. Cell Transplant. 2019; 28(7):943-954. PMC: 6719494. DOI: 10.1177/0963689718821686. View

13.

Fujita T, Umeki H, Shimura H, Kugumiya K, Shiga K . Effect of group culture and embryo-culture conditioned medium on development of bovine embryos. J Reprod Dev. 2005; 52(1):137-42. DOI: 10.1262/jrd.16084. View

14.

van den Hurk R, Zhao J . Formation of mammalian oocytes and their growth, differentiation and maturation within ovarian follicles. Theriogenology. 2005; 63(6):1717-51. DOI: 10.1016/j.theriogenology.2004.08.005. View

15.

Combelles C, Gupta S, Agarwal A . Could oxidative stress influence the in-vitro maturation of oocytes?. Reprod Biomed Online. 2009; 18(6):864-80. PMC: 3235363. DOI: 10.1016/s1472-6483(10)60038-7. View

16.

Jatesada J, Elisabeth P, Anne-Marie D . Seminal plasma did not influence the presence of transforming growth factor-β1, interleukine-10 and interleukin-6 in porcine follicles shortly after insemination. Acta Vet Scand. 2013; 55:66. PMC: 3850961. DOI: 10.1186/1751-0147-55-66. View

17.

Sirotkin A, Dukesova J, Makarevich A, Kubek A, Bulla J . Evidence that growth factors IGF-I, IGF-II and EGF can stimulate nuclear maturation of porcine oocytes via intracellular protein kinase A. Reprod Nutr Dev. 2001; 40(6):559-69. DOI: 10.1051/rnd:2000137. View

18.

Zimmermann R, Hartman T, Kavic S, Pauli S, Bohlen P, Sauer M . Vascular endothelial growth factor receptor 2-mediated angiogenesis is essential for gonadotropin-dependent follicle development. J Clin Invest. 2003; 112(5):659-69. PMC: 182212. DOI: 10.1172/JCI18740. View

19.

Sugiura K, Su Y, Diaz F, Pangas S, Sharma S, Wigglesworth K . Oocyte-derived BMP15 and FGFs cooperate to promote glycolysis in cumulus cells. Development. 2007; 134(14):2593-603. DOI: 10.1242/dev.006882. View

20.

Yang X, Wang Y, Qu X, Duan C, Xu J . [Possible mechanism of endothelial progenitor cells in the development of rat choroidal neovascularization]. Zhonghua Yan Ke Za Zhi. 2012; 48(7):610-4. View