Evaluation of the Selectivity of G-quadruplex Ligands in Living Cells with a Small Molecule Fluorescent Probe

Overview

Journal Anal Chim Acta X

Specialty Chemistry

Date 2020 Oct 29

PMID 33117978

Citations 2

Authors

Suge Zhang

Hongxia Sun

Dawei Yang

Yan Liu

Xiufeng Zhang

Hongbo Chen

Qian Li

Aijiao Guan

Yalin Tang

Affiliations

Soon will be listed here.

Abstract

G-quadruplex has been an emerging target for drug design due to its physiologically important roles in oncology. A number of quadruplex-interactive ligands have been developed by synthetic and medicinal chemists over the past decades. However, the great challenge still remains that the method for detecting the specific targeting of these ligands to the G-quadruplex structures in cells is still lacking. Herein, a detection system for directly identifying the specific targeting of a ligand to DNA G-quadruplexes in cells was constructed by using a small-molecular fluorescent probe (IMT) as a fluorescent indicator. Four typical ligands have been successfully evaluated, demonstrating the promising application of this detection system in the screening and evaluation of quadruplex-specific therapeutic agents.

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