Biophysical and Lipidomic Biomarkers of Cardiac Remodeling Post-Myocardial Infarction in Humans

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2020 Oct 27

PMID 33105904

Citations 10

Authors

Valerie Samouillan

Ignacio Miguel Martinez de Lejarza Samper

Aleyda Benitez Amaro

David Vilades

Jany Dandurand

Josefina Casas

Esther Jorge

David de Gonzalo Calvo

Alberto Gallardo

Enrique Lerma

Jose Maria Guerra Ramos

Francesc Carreras

Ruben Leta

Vicenta Llorente Cortes

Affiliations

Soon will be listed here.

Abstract

Few studies have analyzed the potential of biophysical parameters as markers of cardiac remodeling post-myocardial infarction (MI), particularly in human hearts. Fourier transform infrared spectroscopy (FTIR) illustrates the overall changes in proteins, nucleic acids and lipids in a single signature. The aim of this work was to define the FTIR and lipidomic pattern for human left ventricular remodeling post-MI. A total of nine explanted hearts from ischemic cardiomyopathy patients were collected. Samples from the right ventricle (RV), left ventricle (LV) and infarcted left ventricle (LV INF) were subjected to biophysical (FTIR and differential scanning calorimetry, DSC) and lipidomic (liquid chromatography-high-resolution mass spectrometry, LC-HRMS) studies. FTIR evidenced deep alterations in the myofibers, extracellular matrix proteins, and the hydric response of the LV INF compared to the RV or LV from the same subject. The lipid and esterified lipid FTIR bands were enhanced in LV INF, and both lipid indicators were tightly and positively correlated with remodeling markers such as collagen, lactate, polysaccharides, and glycogen in these samples. Lipidomic analysis revealed an increase in several species of sphingomyelin (SM), hexosylceramide (HexCer), and cholesteryl esters combined with a decrease in glycerophospholipids in the infarcted tissue. Our results validate FTIR indicators and several species of lipids as useful markers of left ventricular remodeling post-MI in humans.

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References

Lewis G, Asnani A, Gerszten R . Application of metabolomics to cardiovascular biomarker and pathway discovery. J Am Coll Cardiol. 2008; 52(2):117-23. PMC: 3204897. DOI: 10.1016/j.jacc.2008.03.043. View

Yang T, Weng S, Zheng N, Pan Q, Cao H, Liu L . Histopathology mapping of biochemical changes in myocardial infarction by Fourier transform infrared spectral imaging. Forensic Sci Int. 2011; 207(1-3):e34-9. DOI: 10.1016/j.forsciint.2010.12.005. View

Zohdi V, Wood B, Pearson J, Bambery K, Black M . Evidence of altered biochemical composition in the hearts of adult intrauterine growth-restricted rats. Eur J Nutr. 2012; 52(2):749-58. DOI: 10.1007/s00394-012-0381-x. View

Wang D, Toledo E, Hruby A, Rosner B, Willett W, Sun Q . Plasma Ceramides, Mediterranean Diet, and Incident Cardiovascular Disease in the PREDIMED Trial (Prevención con Dieta Mediterránea). Circulation. 2017; 135(21):2028-2040. PMC: 5496817. DOI: 10.1161/CIRCULATIONAHA.116.024261. View

Hazen S, Ford D, Gross R . Activation of a membrane-associated phospholipase A2 during rabbit myocardial ischemia which is highly selective for plasmalogen substrate. J Biol Chem. 1991; 266(9):5629-33. View

Hadas Y, Vincek A, Youssef E, Zak M, Chepurko E, Sultana N . Altering Sphingolipid Metabolism Attenuates Cell Death and Inflammatory Response After Myocardial Infarction. Circulation. 2020; 141(11):916-930. PMC: 7135928. DOI: 10.1161/CIRCULATIONAHA.119.041882. View

Wang H, Liu C, Wu H, Kuo J . Direct profiling of phospholipids and lysophospholipids in rat brain sections after ischemic stroke. Rapid Commun Mass Spectrom. 2010; 24(14):2057-64. DOI: 10.1002/rcm.4620. View

Bozkurt O, Severcan M, Severcan F . Diabetes induces compositional, structural and functional alterations on rat skeletal soleus muscle revealed by FTIR spectroscopy: a comparative study with EDL muscle. Analyst. 2010; 135(12):3110-9. DOI: 10.1039/c0an00542h. View

Opie L, Commerford P, Gersh B, Pfeffer M . Controversies in ventricular remodelling. Lancet. 2006; 367(9507):356-67. DOI: 10.1016/S0140-6736(06)68074-4. View

10.

Dang V, Huang A, Werstuck G . Untargeted Metabolomics in the Discovery of Novel Biomarkers and Therapeutic Targets for Atherosclerotic Cardiovascular Diseases. Cardiovasc Hematol Disord Drug Targets. 2018; 18(3):166-175. DOI: 10.2174/1871529X18666180420170108. View

11.

Weber M, Mera P, Casas J, Salvador J, Rodriguez A, Alonso S . Liver CPT1A gene therapy reduces diet-induced hepatic steatosis in mice and highlights potential lipid biomarkers for human NAFLD. FASEB J. 2020; 34(9):11816-11837. DOI: 10.1096/fj.202000678R. View

12.

Samouillan V, Revuelta-Lopez E, Soler-Botija C, Dandurand J, Benitez-Amaro A, Nasarre L . Conformational and thermal characterization of left ventricle remodeling post-myocardial infarction. Biochim Biophys Acta Mol Basis Dis. 2017; 1863(6):1500-1509. DOI: 10.1016/j.bbadis.2017.02.025. View

13.

Chen W, Chao Y, Chang W, Chan J, Hsu Y . Phosphatidylglycerol Incorporates into Cardiolipin to Improve Mitochondrial Activity and Inhibits Inflammation. Sci Rep. 2018; 8(1):4919. PMC: 5861085. DOI: 10.1038/s41598-018-23190-z. View

14.

Drevinge C, Karlsson L, Stahlman M, Larsson T, Sundelin J, Grip L . Cholesteryl esters accumulate in the heart in a porcine model of ischemia and reperfusion. PLoS One. 2013; 8(4):e61942. PMC: 3637450. DOI: 10.1371/journal.pone.0061942. View

15.

Petibois C, Gouspillou G, Wehbe K, Delage J, Deleris G . Analysis of type I and IV collagens by FT-IR spectroscopy and imaging for a molecular investigation of skeletal muscle connective tissue. Anal Bioanal Chem. 2006; 386(7-8):1961-6. DOI: 10.1007/s00216-006-0828-0. View

16.

Laaksonen R, Ekroos K, Sysi-Aho M, Hilvo M, Vihervaara T, Kauhanen D . Plasma ceramides predict cardiovascular death in patients with stable coronary artery disease and acute coronary syndromes beyond LDL-cholesterol. Eur Heart J. 2016; 37(25):1967-76. PMC: 4929378. DOI: 10.1093/eurheartj/ehw148. View

17.

Castellano J, Aledo R, Sendra J, Costales P, Juan-Babot O, Badimon L . Hypoxia stimulates low-density lipoprotein receptor-related protein-1 expression through hypoxia-inducible factor-1α in human vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 2011; 31(6):1411-20. DOI: 10.1161/ATVBAHA.111.225490. View

18.

Hernandez-Corbacho M, Jenkins R, Clarke C, Hannun Y, Obeid L, Snider A . Accumulation of long-chain glycosphingolipids during aging is prevented by caloric restriction. PLoS One. 2011; 6(6):e20411. PMC: 3110726. DOI: 10.1371/journal.pone.0020411. View

19.

Zhang D, Fryer R, Hsu A, Zou A, Gross G, Campbell W . Production and metabolism of ceramide in normal and ischemic-reperfused myocardium of rats. Basic Res Cardiol. 2001; 96(3):267-74. DOI: 10.1007/s003950170057. View

20.

Mignolet A, Derenne A, Smolina M, Wood B, Goormaghtigh E . FTIR spectral signature of anticancer drugs. Can drug mode of action be identified?. Biochim Biophys Acta. 2015; 1864(1):85-101. DOI: 10.1016/j.bbapap.2015.08.010. View