Morphological and Molecular Analysis of Henneguya Tietensis N. Sp. (Cnidaria: Myxosporea), Parasitizing the Gill Filaments of Prochilodus Lineatus (Valenciennes, 1837) from Brazil

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2020 Oct 26

PMID 33103215

Citations 3

Authors

Diego Henrique Mirandola Dias Vieira

Luis Filipe Rangel

Vinicius Panciera Tagliavini

Vanessa Doro Abdallah

Maria Joao Santos

Rodney Kozlowiski de Azevedo

Affiliations

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Abstract

The present study describes Henneguya tietensis n. sp. parasitizing Prochilodus lineatus (Valenciennes, 1837) from Brazil, through morphological and molecular analysis. Myxospores were found in the gill tissue of P. lineatus with a prevalence of 10%. The myxospores consisted of two elongate, elliptical shell valves each with a long, tapering caudal appendage. The morphology was consistent with Henneguya and the myxospore measured as follows (mean ± SD): total length 55.5 ± 2.1 μm, body length 16.2 ± 1.1 μm, body width 5.5 ± 0.1 μm, caudal appendages length 39.0 ± 2.0 μm. The polar capsules were 7.3 ± 0.2 μm long, 1.7 ± 0.2 μm wide and contained a polar filament coiled 11 to 13 turns. Histological analysis showed the plasmodia developing in the middle region of each gill filament and caused a focally extensive distension by forming a space-occupying mass within the gill filament. The ultrastructural analysis allowed the observation of the presence of mature myxospores throughout the plasmodium. Phylogenetic analysis with the SSU rDNA gene showed Henneguya tietensis n. sp. as a sister species of the subclade formed by Henneguya piaractus and Henneguya brachypomus that infect fishes of the genus Piaractus. The genetically closest species was H. piaractus, which showed a similarity of 82.4%. Using molecular and morphological characterization, the myxozoan parasite described herein represents a new species of the genus Henneguya.

Citing Articles

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Diversity of myxozoans parasitizing the catfish Rhamdia quelen (Siluriformes: Heptapteridae), in southeastern Brazil, based on morphological and molecular evidence.

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