Survival of Recombinant Monoclonal and Naturally-occurring Human Milk Immunoglobulins A and G Specific to Respiratory Syncytial Virus F Protein Across Simulated Human Infant Gastrointestinal Digestion

Overview

Journal J Funct Foods

Publisher Elsevier

Date 2020 Oct 26

PMID 33101461

Citations 2

Authors

Jiraporn Lueangsakulthai

Baidya Nath P Sah

Brian P Scottoline

David C Dallas

Affiliations

Soon will be listed here.

Abstract

To help rationally design an antibody for oral administration, we examined how different isotypes (IgG, IgA and sIgA) with the same variable sequence affect antibody stability across digestion. We compared the degradation of recombinant palivizumab (IgG1), and recombinant IgA and sIgA versions of palivizumab spiked in human milk to the degradation of naturally-occurring anti-respiratory syncytial virus (RSV) sIgA/IgA and IgG in human milk from four donors across gastric and intestinal phases of an model of infant digestion via a validated RSV F protein ELISA. Palivizumab IgG and IgA formats were less stable than the sIgA version after complete simulated gastrointestinal digestion: palivizumab IgG, IgA and sIgA decreased across complete simulated gastrointestinal digestion by 55%, 48% and 28%, respectively. Naturally-occurring RSV F protein-specific IgG was stable across digestion, whereas naturally-occurring sIgA/IgA was stable in the gastric phase but decreased 33% in the intestinal phase of simulated digestion.

Citing Articles

Complementary measurement of nontyphoidal specific IgG and IgA antibodies in oral fluid and serum.

Elias S, Muthumbi E, Mwanzu A, Wanjiku P, Mutiso A, Simon R Heliyon. 2023; 9(1):e12071.

PMID: 36704288 PMC: 9871079. DOI: 10.1016/j.heliyon.2022.e12071.

Survival of Vaccine-Induced Human Milk SARS-CoV-2 IgG, IgA and SIgA Immunoglobulins across Simulated Human Infant Gastrointestinal Digestion.

Pieri M, Maniori M, Shahabian L, Kanaan E, Paphiti-Demetriou I, Pipis S Nutrients. 2022; 14(16).

PMID: 36014873 PMC: 9415426. DOI: 10.3390/nu14163368.

References

Reilly R, Domingo R, Sandhu J . Oral delivery of antibodies. Future pharmacokinetic trends. Clin Pharmacokinet. 1997; 32(4):313-23. DOI: 10.2165/00003088-199732040-00004. View

Roos N, Mahe S, Benamouzig R, SICK H, Rautureau J, Tome D . 15N-labeled immunoglobulins from bovine colostrum are partially resistant to digestion in human intestine. J Nutr. 1995; 125(5):1238-44. DOI: 10.1093/jn/125.5.1238. View

Munoz F, Piedra P, Glezen W . Safety and immunogenicity of respiratory syncytial virus purified fusion protein-2 vaccine in pregnant women. Vaccine. 2003; 21(24):3465-7. DOI: 10.1016/s0264-410x(03)00352-9. View

Stensballe L, Ravn H, Kristensen K, Meakins T, Aaby P, Simoes E . Seasonal variation of maternally derived respiratory syncytial virus antibodies and association with infant hospitalizations for respiratory syncytial virus. J Pediatr. 2009; 154(2):296-8. DOI: 10.1016/j.jpeds.2008.07.053. View

Stoll B, Lee F, Hale E, Schwartz D, Holmes R, Ashby R . Immunoglobulin secretion by the normal and the infected newborn infant. J Pediatr. 1993; 122(5 Pt 1):780-6. DOI: 10.1016/s0022-3476(06)80026-0. View

HILPERT H, Brussow H, MIETENS C, Sidoti J, Lerner L, Werchau H . Use of bovine milk concentrate containing antibody to rotavirus to treat rotavirus gastroenteritis in infants. J Infect Dis. 1987; 156(1):158-66. DOI: 10.1093/infdis/156.1.158. View

Moldt B, Saye-Francisco K, Schultz N, Burton D, Hessell A . Simplifying the synthesis of SIgA: combination of dIgA and rhSC using affinity chromatography. Methods. 2013; 65(1):127-32. PMC: 3858571. DOI: 10.1016/j.ymeth.2013.06.022. View

Hiatt A, Bohorova N, Bohorov O, Goodman C, Kim D, Pauly M . Glycan variants of a respiratory syncytial virus antibody with enhanced effector function and in vivo efficacy. Proc Natl Acad Sci U S A. 2014; 111(16):5992-7. PMC: 4000855. DOI: 10.1073/pnas.1402458111. View

Chirico G, Marzollo R, Cortinovis S, Fonte C, Gasparoni A . Antiinfective properties of human milk. J Nutr. 2008; 138(9):1801S-1806S. DOI: 10.1093/jn/138.9.1801S. View

10.

Mazur N, Horsley N, Englund J, Nederend M, Magaret A, Kumar A . Breast Milk Prefusion F Immunoglobulin G as a Correlate of Protection Against Respiratory Syncytial Virus Acute Respiratory Illness. J Infect Dis. 2018; 219(1):59-67. PMC: 6284547. DOI: 10.1093/infdis/jiy477. View

11.

Huang J, Kailemia M, Goonatilleke E, Parker E, Hong Q, Sabia R . Quantitation of human milk proteins and their glycoforms using multiple reaction monitoring (MRM). Anal Bioanal Chem. 2016; 409(2):589-606. PMC: 5650958. DOI: 10.1007/s00216-016-0029-4. View

12.

Dougan G, Huett A, Clare S . Vaccines against human enteric bacterial pathogens. Br Med Bull. 2002; 62:113-23. DOI: 10.1093/bmb/62.1.113. View

13.

Kotloff K, Blackwelder W, Nasrin D, Nataro J, Farag T, van Eijk A . The Global Enteric Multicenter Study (GEMS) of diarrheal disease in infants and young children in developing countries: epidemiologic and clinical methods of the case/control study. Clin Infect Dis. 2012; 55 Suppl 4:S232-45. PMC: 3502307. DOI: 10.1093/cid/cis753. View

14.

Goonatilleke E, Huang J, Xu G, Wu L, Smilowitz J, German J . Human Milk Proteins and Their Glycosylation Exhibit Quantitative Dynamic Variations during Lactation. J Nutr. 2019; 149(8):1317-1325. PMC: 6686052. DOI: 10.1093/jn/nxz086. View

15.

Eibl M, Wolf H, Furnkranz H, Rosenkranz A . Prevention of necrotizing enterocolitis in low-birth-weight infants by IgA-IgG feeding. N Engl J Med. 1988; 319(1):1-7. DOI: 10.1056/NEJM198807073190101. View

16.

Liu H, Manuilov A, Chumsae C, Babineau M, Tarcsa E . Quantitation of a recombinant monoclonal antibody in monkey serum by liquid chromatography-mass spectrometry. Anal Biochem. 2011; 414(1):147-53. DOI: 10.1016/j.ab.2011.03.004. View

17.

Olchanski N, Hansen R, Pope E, DCruz B, Fergie J, Goldstein M . Palivizumab Prophylaxis for Respiratory Syncytial Virus: Examining the Evidence Around Value. Open Forum Infect Dis. 2018; 5(3):ofy031. PMC: 5833316. DOI: 10.1093/ofid/ofy031. View

18.

Nguyen T, Bhandari B, Cichero J, Prakash S . Gastrointestinal digestion of dairy and soy proteins in infant formulas: An in vitro study. Food Res Int. 2017; 76(Pt 3):348-358. DOI: 10.1016/j.foodres.2015.07.030. View

19.

Lawrence R, Pane C . Human breast milk: current concepts of immunology and infectious diseases. Curr Probl Pediatr Adolesc Health Care. 2006; 37(1):7-36. DOI: 10.1016/j.cppeds.2006.10.002. View

20.

Sparrow E, Friede M, Sheikh M, Torvaldsen S . Therapeutic antibodies for infectious diseases. Bull World Health Organ. 2017; 95(3):235-237. PMC: 5328111. DOI: 10.2471/BLT.16.178061. View