2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-Glucoside Modulated Human Umbilical Vein Endothelial Cells Injury Under Oxidative Stress

Overview

Journal Korean J Physiol Pharmacol

Specialty Pharmacology

Date 2020 Oct 23

PMID 33093269

Citations 2

Authors

Yan Guo

Wenxue Fan

Shuyu Cao

Yuefeng Xie

Jiancong Hong

Huifen Zhou

Haitong Wan

Bo Jin

Affiliations

Soon will be listed here.

Abstract

Endothelial cell injury is a major contributor to cardiovascular diseases. The 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-Glucoside (TSG) contributes to alleviate human umbilical vein endothelial cells (HUVECs) injury through mechanisms still know a little. This study aims to clarify the TSG effects on gene expression (mRNA and microRNA) related to oxidative stress and endoplasmic reticulum stress induced by HO in HUVECs. We found that TSG significantly reduced the death rate of cells and increased intracellular superoxide dismutase activity. At qRT-PCR, experimental data showed that TSG significantly counteracted the expressions of miR-9-5p, miR-16, miR-21, miR-29b, miR-145-5p, and miR-204-5p. Besides, TSG prevented the expression of ATF6 and CHOP increasing. In contrast, TSG promoted the expression of E2F1. In conclusion, our results point to the obvious protective effect of TSG on HUVECs injury induced by HO, and the mechanism may through miR16/ATF6/ E2F1 signaling pathway.

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