Mini-III RNase-based Dual-color System for MRNA Tracking

Overview

Journal Development

Specialty Biology

Date 2020 Oct 23

PMID 33093152

Citations 1

Authors

Lin Zhang

Luxi Chen

Jing Chen

Weimin Shen

Anming Meng

Affiliations

Soon will be listed here.

Abstract

Mini-III RNase (mR3), a member of RNase III endonuclease family, can bind to and cleave double-stranded RNAs (dsRNAs). Inactive mR3 protein without the α5β-α6 loop loses the dsRNA cleavage activity, but retains dsRNA binding activity. Here, we establish an inactive mR3-based non-engineered mR3/dsRNA system for RNA tracking in zebrafish embryos. binding experiments show that inactive mR3 (dSmR3) protein possesses the highest binding affinity with dsRNAs among mR3s from other related species, and its binding property is retained in zebrafish embryos. Combined with a fluorescein-labeled antisense RNA probe recognizing the target mRNAs, dSmR3 tagged with a nuclear localization sequence and a fluorescent protein could allow visualization of the dynamics of endogenous target mRNAs. The dSmR3/antisense probe dual-color system provides a new approach for tracking non-engineered RNAs in real-time, which will help understand how endogenous RNAs dynamically move during embryonic development.

Citing Articles

Single-cell transcriptional dynamics in a living vertebrate.

Eck E, Moretti B, Schlomann B, Bragantini J, Lange M, Zhao X bioRxiv. 2024; .

PMID: 38260569 PMC: 10802376. DOI: 10.1101/2024.01.03.574108.

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