Transcriptome Analysis of Reveals Molecular Signature of Saccharide Impact on Acarbose Biosynthesis

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2020 Oct 22

PMID 33088668

Citations 2

Authors

Chun-Yue Weng

Li-Zhen Shi

Ya-Jun Wang

Yu-Guo Zheng

Affiliations

Soon will be listed here.

Abstract

Different carbon sources lead to differential acarbose production in . To uncover the underlying differentiation in the context of genes and pathways, we performed transcriptome sequencing of ZJB-03852 grown on different saccharides, such as glucose, maltose, or the saccharide complex consisting of glucose plus maltose. The differentially expressed genes were classified into GO (gene ontology) terms and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways for functional annotations. Key enriched modules were uncovered. Our data revealed that both maltose and its complex with glucose gave improved acarbose titer. Sugar transportation, cytochrome oxidase, protein synthesis and amino acid metabolism modules were enriched under the saccharide complex condition, while ferritin metabolism gene expressions were enriched in the glucose medium. Our results provided the foundation for uncovering the mechanism of carbon source on acarbose production in .

Citing Articles

Enhancement of acarbose production by genetic engineering and fed-batch fermentation strategy in Actinoplanes sp. SIPI12-34.

Li Z, Yang S, Zhang Z, Wu Y, Tang J, Wang L Microb Cell Fact. 2022; 21(1):240.

PMID: 36419063 PMC: 9685945. DOI: 10.1186/s12934-022-01969-0.

Gene Cascade Shift and Pathway Enrichment in Rat Kidney Induced by Acarbose Through Comparative Analysis.

Weng C, Zhu M, Dai K, Mi Z, Wang Y, Liu Z Front Bioeng Biotechnol. 2021; 9:659700.

PMID: 34095098 PMC: 8176958. DOI: 10.3389/fbioe.2021.659700.

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