Liquid Drop of DNA Libraries Reveals Total Genome Information

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2020 Oct 22

PMID 33087570

Citations 7

Authors

Stanislav S Terekhov

Igor E Eliseev

Leyla A Ovchinnikova

Marsel R Kabilov

Andrey D Prjibelski

Alexey E Tupikin

Ivan V Smirnov

Alexey A Belogurov Jr

Konstantin V Severinov

Yakov A Lomakin

Sidney Altman

Alexander G Gabibov

Affiliations

Soon will be listed here.

Abstract

Conventional "bulk" PCR often yields inefficient and nonuniform amplification of complex templates in DNA libraries, introducing unwanted biases. Amplification of single DNA molecules encapsulated in a myriad of emulsion droplets (emulsion PCR, ePCR) allows the mitigation of this problem. Different ePCR regimes were experimentally analyzed to identify the most robust techniques for enhanced amplification of DNA libraries. A phenomenological mathematical model that forms an essential basis for optimal use of ePCR for library amplification was developed. A detailed description by high-throughput sequencing of amplified DNA-encoded libraries highlights the principal advantages of ePCR over bulk PCR. ePCR outperforms PCR, reduces gross DNA errors, and provides a more uniform distribution of the amplified sequences. The quasi single-molecule amplification achieved via ePCR represents the fundamental requirement in case of complex DNA templates being prone to diversity degeneration and provides a way to preserve the quality of DNA libraries.

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