Development of PCR-Based Molecular Marker for Detection of Pv. Race 6, the Causative Agent of Black Rot of Brassicas

Overview

Journal Plant Pathol J

Date 2020 Oct 21

PMID 33082726

Citations 2

Authors

Khandker Shazia Afrin

Md Abdur Rahim

Mehede Hassan Rubel

Jong-In Park

Hee-Jeong Jung

Hoy-Taek Kim

Ill-Sup Nou

Affiliations

Soon will be listed here.

Abstract

pv. (), the pathogen of black rot which is the most destructive disease of Brassica vegetables throughout the world. Here, we reported two novel sequence-characterized amplified region (SCAR) markers (i.e., XccR6-60 and XccR6-67) for the detection of race 6 via re-alignment of the complete genome sequences of races/strains/pathovars. The specificity of SCAR primer sets was verified by mean of PCR amplification using the genomic DNA template of races/strains/pathovars and two other plant infecting bacterial strains. The PCR result revealed that the XccR6-60 and XccR6-67 primer sets amplified 692-bp and 917-bp DNA fragments, respectively, specifically from race 6, while no visible amplification was detected in other samples. In addition, the SCAR primers were highly sensitive and can detect from a very low concentration of genomic DNA of race 6. However, the complete genome sequence of race 6 is not yet publicly available. Therefore, the cloning and sequencing of XccR6-60 and XccR6-67 fragments from race 6 provide more evidence of the specificity of these markers. These results indicated that the newly developed SCAR markers can successfully, effectively and rapidly detect race 6 from other races/strains/pathovars as well as other plant pathogenic bacteria. This is the first report for race-specific molecular markers for race 6.

Citing Articles

Molecular Marker Development for the Rapid Differentiation of Black Rot Causing Xanthomonas campestris pv. campestris Race 7.

Kim Y, Mao S, Sahu N, Somaddar U, Kim H, Watanabe M Plant Pathol J. 2023; 39(5):494-503.

PMID: 37817495 PMC: 10580059. DOI: 10.5423/PPJ.OA.07.2023.0102.

The type-III effectors-based multiplex PCR for detection of pv. causing black rot disease in crucifer crops.

Singh D, Kesharwani A, Avasthi A 3 Biotech. 2023; 13(8):272.

PMID: 37449249 PMC: 10335992. DOI: 10.1007/s13205-023-03691-z.

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