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CD5-positive B Cells After T Cell Depleted Bone Marrow Transplantation

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Date 1987 Jun 1
PMID 3308220
Citations 1
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Abstract

CD5 is an antigen normally found on T cells and on a minority subpopulation of B cells in fetal spleen and tonsil and on the majority of cells in B-chronic lymphocytic leukaemia. Recent reports described the occurrence of large numbers of CD5-positive B cells in the peripheral blood after bone marrow transplantation (BMT). We examined the peripheral blood for CD5-positive B cells in 21 patients who underwent allogeneic BMT for leukaemia with marrow first depleted of T cells using anti-T monoclonal antibodies and complement mediated lysis. Twenty-six samples were obtained from patients 24-644 days after BMT and examined for the existence of blood-borne CD5-positive B cells by immunofluorescence analysis microscopically and by flow cytometry. The number of CD5-positive B cells was consistently lower than 2%. The absence of CD5-positive B cells in this series may be due to the method of T cell depletion of the marrow or to methodological differences in the analysis of the cells.

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Rapid recovery of helper activity following T cell depleted allogeneic marrow transplant.

Wimperis J, Brenner M, Drexler H, Hoffbrand A, Prentice H Clin Exp Immunol. 1987; 69(3):601-10.

PMID: 2959412 PMC: 1542360.

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