Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells Upon In Vitro Contact with Microarc Calcium Phosphate Coating

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Oct 21

PMID 33081386

Citations 3

Authors

Larisa Litvinova

Kristina Yurova

Valeria Shupletsova

Olga Khaziakhmatova

Vladimir Malashchenko

Egor Shunkin

Elena Melashchenko

Natalia Todosenko

Marina Khlusova

Yurii Sharkeev

Ekaterina Komarova

Maria Sedelnikova

Igor Khlusov

Affiliations

Soon will be listed here.

Abstract

The manufacture of biomaterial surfaces with desired physical and chemical properties that can directly induce osteogenic differentiation without the need for biochemical additives is an excellent strategy for controlling the behavior of mesenchymal stem cells (MSCs) in vivo. We studied the cellular and molecular reactions of MSCs to samples with a double-sided calcium phosphate (CaP) coating and an average roughness index (Ra) of 2.4-4.6 µm. The study aimed to evaluate the effect of a three-dimensional matrix on the relative mRNA expression levels of genes associated with the differentiation and maturation of MSCs toward osteogenesis (, , , , and ) under conditions of distant interaction in vitro. Correlations were revealed between the mRNA expression of some osteogenic and cytokine/chemokine genes and the secretion of cytokines and chemokines that may potentiate the differentiation of cells into osteoblasts, which indicates the formation of humoral components of the extracellular matrix and the creation of conditions supporting the establishment of hematopoietic niches.

Citing Articles

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Medical Genetics, Genomics and Bioinformatics Aid in Understanding Molecular Mechanisms of Human Diseases.

Orlov Y, Anashkina A, Klimontov V, Baranova A Int J Mol Sci. 2021; 22(18).

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Amorphous-Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2/CD3/CD28 Antibodies.

Sharkeev Y, Komarova E, Chebodaeva V, Sedelnikova M, Zakharenko A, Golokhvast K Materials (Basel). 2021; 14(13).

PMID: 34279263 PMC: 8269898. DOI: 10.3390/ma14133693.

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